RT Journal Article SR Electronic T1 Normal antioxidative enzyme activities in several genes are associated with less bronchial hyperresponsiveness (BHR) among young Danes JF European Respiratory Journal JO Eur Respir J FD European Respiratory Society SP P3393 VO 40 IS Suppl 56 A1 Vivi Schlünssen A1 Tine Halsen Malling A1 Torben Sigsgaard A1 Charlotte Brasch-Andersen A1 Gert Thomsen A1 David Sherson A1 Lars Skadhauge A1 Jesper Bælum A1 Øyvind Omland YR 2012 UL http://erj.ersjournals.com/content/40/Suppl_56/P3393.abstract AB BACKGOUND AND AIMBHR might be associated to the oxidative defense. We hypothesize that genotypes coding for normal antioxidative enzyme activity (AEA) influence the occurrence of BHR.METHODSIn a cross sectional study 7,271 subjects aged 20-44 year (73% response rate) were recruited using an asthma screening questionnaire. All subjects with asthma (n = 460) and a 20% random sample (n= 728) were clinically investigated, including a bronchial provocation test, skin prick test (SPT) with 13 aeroallergens, and a blood sample. A bronchial provocation test was available for 956 subjects, and BHR was defined as at least 20% drop in baseline FEV1. Variants in the following genes were genotyped: Glutathione peroxidase, GPX1 (Pro198Leu, rs1050450), manganese superoxide dismutase, SOD2 (Ala16Val, rs4880) and 3 glutathione S-transferases; GSTP1 (Ile105Val, rs1695), GSTT1 (gene copy nr) and GSTM1 (gene copy nr).RESULTSThe frequency of BHR was 12.8% in the random sample and 42.6% in the asthma sample. Log. reg. models showed a neg. association between being BHR and having at least 4 genotypes coding for normal AEA compared to no normal genotype, OR 0.24 (0.06-0.94) adj. for smoking, FEV1, sex, atopy, height2 and SPT-size of HDM. The result were similar after further adjustments for BMI, county and sample (random/case), OR 0.25 (0.06-1.06). ORs for BHR were decreased for 1 – 3 genotypes with normal AEA compared to no normal genotype, but not significantly so and no clear dose-response relations were seen.CONCLUSIONThis study suggests, that a combined effect of several genotypes coding for normal AEA might be a protective factor for BHR among young adults.