PT  - JOURNAL ARTICLE
AU  - Jianmei Qian
AU  - Chunxue Bai
AU  - Guifang Wang
TI  - Study on human tracheal bronchial epithelial cells secreting cytokines in vitro by nontypeable haemophilus influenzae
DP  - 2012 Sep 01
TA  - European Respiratory Journal
PG  - P2509
VI  - 40
IP  - Suppl 56
4099  - http://erj.ersjournals.com/content/40/Suppl_56/P2509.short
4100  - http://erj.ersjournals.com/content/40/Suppl_56/P2509.full
SO  - Eur Respir J2012 Sep 01; 40
AB  - Obstrucive To study the interaction of nontypeable haemophilus influenzae strain ATCC49247 with primary human bronchial epithelial cells (HBEC) and the NTHi-induced release and expression of proinflammoraty cytokine in the HBEC. Methods HBEC were isolated by using low temperature protease digestion and cultured in serum-free medium. Confluent epithelial cell cultures were incubated with NTHi, NTHi +erythromycin(0.1mg/L), NTHi + erythromycin(10mg/L), NTHi + gentamicin(100mg/L), NTHi+dexamethasone(100uM)and normal untreated control cells after 24h. Release of IL-8 and TNF-a into the supernatant was assayed by enzyme-linked immunosorbent assay; The expression of ICAM-1 was examined by immunohistochemistry staining. Results (1) Some HBECs were transformed and died after 24h. (2) HBEC: NTHi-induced cells released significantly greater amounts of IL-8 and TNF-a (2172.18±131.83, 7.22±2.17 pg/ml, respectively) than normal untreated cells (1115.76±57.63,2.84±1.04pg/ml)(p<0.001). NTHi also significantly increased the total number of of ICAM-1 positive cells from 10±5%(in control untreated cultures) to 80±5%(p<0.001). Similarly, incubation of HBEC with 0.1mg/L erythromycin and gentamicin significantly induced release of IL-8, TNF-a and the expression of ICAM-1, which was blocked by 10mg/L erythromycin and 100uM dexamethasone. Conclusions HBEC can release IL-8 and TNF-a. NTHi may increase significantly release and expression of proinflammatory cytokine. Gentamicin have no anti-inflammatory effects. Erythromycin may have anti-inflammatory effects. Dexamethasone has distinct anti-inflammatory effects.