RT Journal Article
SR Electronic
T1 Adipose stem cell therapy in mice on repetitive intratracheal bleomycin induced pulmonary fibrosis
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP P1773
VO 40
IS Suppl 56
A1 Sang Hoon Lee
A1 Eun Joo Lee
A1 Seung Hyeun Lee
A1 Gyu Young Hur
A1 Sung Yong Lee
A1 Sang Yeub Lee
A1 Je Hyeong Kim
A1 Jae Jeong Shim
A1 Chol Shin
A1 Kwang Ho In
A1 Kyung Ho Kang
A1 Chang Sub Uhm
A1 Han-Kyeom Kim
A1 Kyung-Sook Yang
A1 Sanghoon Park
A1 Hyun Soo Kim
A1 Yong Man Kim
YR 2012
UL http://erj.ersjournals.com/content/40/Suppl_56/P1773.abstract
AB Rationale: Adipose-derived stem cells are detectable in the parenchyma and large airways of lungs, and associated with reduced inflammatory infiltration and lung cell death.Objectives: We hypothesized that adipose stem cells would ameliorate lung fibrosis induced repetitive intratracheal bleomycin instillation.Methods: Male 8-week-old C57BL/6J mice (control group n=10, bleomycin only group n=20, bleomycin plus adipose stem cell group n=10) were used. Eighth biweekly dose of bleomycin was injected intratracheally via an intubation procedure at dose of 0.04 units in a total volume of 100 μl of sterile saline. Human ASC (6×105 cells) were administered systemically via intraperitoneal injection every other week during the last 2 months of the 4- month bleomycin exposure. Lungs were evaluated for fibrosis and collagen content. Bronchoalveolar lavage (BAL) was performed for cell counts.Measurements and Main Results: Evaluation of lung histology from mice receiving repetitive dosing revealed patchy distributive lung fibrosis and extracellular matrix deposition based on trichrome blue collagen staining. Furthermore, alveolar ducts were increased in size and proliferated with several Clara cells and cuboidal epithelial cells hyperplasia with peri-alveolar ducts inflammatory infiltrations. These finding was ameliorated by adipose stem cells administration.Conclusions: These results suggest a useful therapeutic effect of adipose stem cells on pulmonary fibrosis induced by repetitive bleomycin administration. Further studies are needed to evaluate the efficacy of adipose stem cells for the treatment of human IPF.