TY - JOUR T1 - Alveolar macrophage activity in experimentally induced pneumonia JF - European Respiratory Journal JO - Eur Respir J VL - 40 IS - Suppl 56 SP - P3778 AU - Usman Farkhutdinov Y1 - 2012/09/01 UR - http://erj.ersjournals.com/content/40/Suppl_56/P3778.abstract N2 - Alveolar macrophages (AM) play an important role in lung inflammation.The aim was to study the AM state in experimentally induced pneumonia (PN).Methods: PN was induced in 120 white rats by transthoracic injection of pneumococcus culture. The animals were sacrificed at 1, 3, 7 day of PN course. The content of malonic dialdehyde (MDA) was determined in the animal lungs. The production of active oxygen forms (AOF) by alveolar macrophages (AM) was determined by nitroblue tetrazolium (NBT) test. In addition phagocytic activity (PhA) of AM has been assessed, morphological investigations were performed.Results The development of PN in rats was accompanied by increase of MDA content in the lungs, by increase of NBT-test values and PhA of AM. The NBT values of AM correlated directly to MDA content in the lungs. Due to morphological changes the animals were divided into 2 groups. In both groups the level of MDA in the lungs remained high. In the 1st group of rats on the 3, 7 days NBT values and PhA of AM were found to be increased, infiltration changes were abundant in the lungs. The rats of the 2nd group have demonstrated lowering of NBT values and PhA of AM, destructive changes in the lungs.Conclusion: AOF produced by AM initiated free radical processes and lung inflammation in experimentally induced PN. Decreased production of AOF and reduction of PhA of AM demonstrated the inflammatory complications. ER -