PT  - JOURNAL ARTICLE
AU  - Grant Theron
AU  - Jonny Peter
AU  - Laura Lenders
AU  - Richard van-Zyl Smit
AU  - Richard Meldua
AU  - Ureshnie Govender
AU  - Keertan Dheda
TI  - Correlation of Mycobacterium tuberculosis ¬-specific and non-specific quantitative T cell IFN-γ responses with mycobacillary load in a HIV-prevalent high burden setting
DP  - 2012 Sep 01
TA  - European Respiratory Journal
PG  - P2519
VI  - 40
IP  - Suppl 56
4099  - http://erj.ersjournals.com/content/40/Suppl_56/P2519.short
4100  - http://erj.ersjournals.com/content/40/Suppl_56/P2519.full
SO  - Eur Respir J2012 Sep 01; 40
AB  - Background: Measures of bacillary load in patients with tuberculosis (TB) may be useful for predicting and monitoring response to treatment. The relationship between quantitative T-cell responses and mycobacterial load is poorly studied. We hypothesised that, in a high burden setting, the magnitude of mycobacterial antigen-specific and non-specific T-cell IFN-γ responses would correlate with (a) bacterial load and (b) culture conversion in patients undergoing treatment.Methods: We compared the magnitude of purified-protein-derivative (PPD) and RD1-specific (TSPOT.TB and QFT-GIT) peripheral blood IFN-γ T-cell responses with associates of sputum bacillary load [liquid culture time-to-positivity, smear-microscopy grade, Xpert-MTB/RIF Ct values, and the presence of cavities on a chest radiograph] in 513 individuals with suspected TB in Cape Town, South Africa. Serial IGRA responses were evaluated at 2 (n=35) and 6 months (n=13) post-treatment initiation.Results: PPD and RD1-specific IFN-γ responses were not associated with culture TTP (p-values for TSPOT.TB, QFT-GIT and PPD of 0.11, 0.07 and 0.09), smear-grade (0.42, 0.09, and 0.85), Ct values (0.70, 0.91, and 0.49) or the presence of cavities on the chest radiograph (0.12, >0.05, and 0.08). 2-month IGRA conversion rates (positive to negative) were negligible [<10% for TSPOT.TB (3/28) and QFT-GIT (1/29)] and lower compared to culture [60% (21/35); p<0.01].Conclusions: In a high-burden setting M.tuberculosis-specific and non-specific antigen-driven IFN-γ responses do not correlate with bacillary load and are not useful for prognostication or treatment monitoring.