TY - JOUR T1 - Organization and results of the EGFR mutation Spanish quality control program JF - European Respiratory Journal JO - Eur Respir J VL - 40 IS - Suppl 56 SP - P3138 AU - José Javier Gómez-Román AU - Sola Gallego Iosu AU - Bárbara Angulo AU - Nicolás Martínez Montserrat AU - Lazuén Fernández Servando Y1 - 2012/09/01 UR - http://erj.ersjournals.com/content/40/Suppl_56/P3138.abstract N2 - There is a need for EGFR mutation performing in non-small cell carcinomas to select the best treatment. One of the most important problems is accuracy of results. The Spanish Society of Pathology has developped a quality control program. We present their structure and results.The organizing laboratory selects four cases tested with two techniques to send them. To simulate bronchoscopic samples tissue microarray punchers are used to construct a paraffin block with five to six 1 mm samples. Four unstained slides are identified and sent. Each laboratory extracts DNA and perform the analysis with their own method. They have to stain one of the sections and to inform about histology and percentage of tumoral cells. A questionary with information about the method, number of analysis performed last year, and the text with all the information they include in clinical practice are analysed by the organizing laboratory. A general and a particular inform are sent to each participant.In the third round 31 spanish centres (25 hospitals, two medicine schools and four private laboratories) sent the complete results. Three mutated (one L858R and two different Del19) and one wild-type adenocarcinomas were selected. No false positive results were reported. There were 11 non-informative and 13 false-negative cases among the 124 analysed. Most of the false-negative results were obtained in centres that used Sanger sequentiation in the L858R case where the percentage of neoplastic cells was small.There is a need to stablish an organized quality control program for molecular testing in lung cancer patients. Pathology societies could be the organizers making routine laboratories perform these tests according to best practises. ER -