TY - JOUR T1 - LSC 2013 abstract - A comparison of responses of dendritic cells derived from cord blood versus adult peripheral blood to TLR ligands and rhinovirus infection JF - European Respiratory Journal JO - Eur Respir J VL - 42 IS - Suppl 57 SP - PP134 AU - Nicole Bedke AU - Emily Swindle AU - Camilia Molnar AU - Patrick Holt AU - Deborah Strickland AU - Graham Roberts AU - Peter Howarth AU - Stephen Holgate AU - Donna Davies Y1 - 2013/09/01 UR - http://erj.ersjournals.com/content/42/Suppl_57/PP134.abstract N2 - Introduction: Atopic asthma in children can develop from early exposure to respiratory viruses and allergens. Dendritic cells (DCs) may play a major part in modulating the immune response to viral infection (Durrani et al 2012). Previous studies have shown that responses of mononuclear cells from cord blood (CB) to TLR ligands differ to those from children and adults (Tulic et al 2011). We characterized the responses of CB-derived DCs (CBDCs) to TLR ligands and rhinovirus (RV) and determined whether they differed from adult peripheral blood monocyte derived DCs (MDDCs).Methods: CD34+ progenitor cells were obtained from CB and expanded in the presence of Flt3L, SCF, TPO, IL-3 and IL-6 followed by 5-6 days in GM-CSF and IL-4. Cells were treated with polyI:C, LPS or RV, and surface markers analysed by flow cytometry. Cytokines were measured by multi-array cytokine assays.Results: CBDCs and adult MDDCs expressed similar levels of CD86 in response to polyI:C and RV. CD80 and HLA-DR were higher in polyI:C-treated adult MDDCs than in CBDCs. CBDCs produced more IFN-γ, TNF-α, IL-1β, IL-6, IL-10, IL-12p70 with polyI:C, and more IL-10 with LPS compared to adult MDDCs. LPS treatment of MDDCs from adults resulted in higher levels of IL-6, IL-8 and TNF-α.Conclusion: Although CBDCs are phenotypically similar to adult MDDCs, they have different cytokines profiles in response to polyI:C and LPS. These differences highlight the need for studying cells from a "naïve" background when trying to understand the early life origins of atopic asthma ER -