TY - JOUR T1 - The percentage of neutrophils expressing TLR4 in induced sputum decreases in asthma not eosinophilic JF - European Respiratory Journal JO - Eur Respir J VL - 42 IS - Suppl 57 SP - P852 AU - Astrid Crespo AU - Eder Mateus AU - Montserrat Torrejón AU - Alicia Belda AU - Jordi Giner AU - Teresa Garriga AU - Silvia Vidal AU - Cándido Juárez AU - Vicente Plaza Y1 - 2013/09/01 UR - http://erj.ersjournals.com/content/42/Suppl_57/P852.abstract N2 - Recently it has involved the role of TLRs (toll-like receptors) in the pathogenesis of asthma.The possible role that TLRs play in IgE-dependent asthma (not eosinophilic) and its recognition as a new endotype asthma, could open new therapeutic expectations. Objective: To analyze the expression of TLR 2 and 4 in monocytes/ macrophages and neutrophils in peripheral blood and induced sputum from asthmatic patients IgE dependent (IgEdep) vs. IgE dependent (IgEnodep). Method: We studied 36 patients with asthma. Asthma ranked IgE dependent (n = 13) and non-dependent (n = 23) according to the level of IgE in the blood. All patients underwent the same day an induced sputum, pulmonary function studies, FeNO, blood total IgE and skin test by prick technique. The cellular composition was analyzed by flow cytometry. Expression of TLRs were analyzed using monoclonal antibodies conjugated with fluorochromes appropriate. Results: Descriptive analysis intergroup and between categorical variables was not significant. The percentage of neutrophils TLR4 + in induced sputum was 1.51% (± 2.98) for asthmatics IgEnodep and 11.17% (±25.15) for asthmatics IgEdep, with a statistically significant correlation (p = 0.006). No significant differences were found among the other groups. Conclusion: The percentage of neutrophils expressing TLR4 in induced sputum decreases in asthma not eosinophilic. This could suggest that in this type of asthma the defense mechanism of neutrophils could be altered by decreasing expression of these receptors on their cell surface. This mechanism would still be defined. Probably the respiratory infections play an important role. ER -