%0 Journal Article %A Alina Alshevskaya %A Fedor Kireev %A Julia Lopatnikova %A Zhanna Laushkina %A Sergey Sennikov %T Expression levels of membrane-bound tumor necrosis factor alpha receptors in active tuberculosis and healthy individuals %D 2013 %J European Respiratory Journal %P P2781 %V 42 %N Suppl 57 %X Background. Tumor necrosis factor alpha (TNF) is critical in control of tuberculosis infection. Its biological effects are mediated by interaction with specific membrane-bound receptors types Iand II (TNFRI/II).Aims and objectives. To analyze whether the percentage of TNFRI+ and TNFRII+ subsets of immunocompetent cells and the number of these receptors differ between patients with active pulmonary tuberculosis (APTB) and healthy individuals.Methods. Peripheral blood was obtained from 25 APTB patients and 150 healthy individuals. The percentage of T cells, B cells and monocytes expressing TNFRI and TNFRII was determined by flow cytometry using phycoerythrin (PE) labelled antibodies to TNFRs (R&D Systems, USA), and the number of TNFRs per cell using QuantiBRITE PE calibration beads (BD, USA).Results. The percentage of TNFRI+ T cells was significantly higher in APTB patients than in healthy individuals (p<0.001), while no differences were shown between groups on the percentage of TNFRI+ B cells and monocytes. The number of TNFRI on T cells and monocytes is siginificantly higher in APTB patients compared with healthy individuals (p<0.05 and p<0.001, respectively). The number of TNFRII receptors on monocytes was significantly higher in APTB patients than in healthy individuals (p<0.001). The number of TNFRII on B cells was significantly higher in healthy individuals (p<0.05).Conclusions. Data obtained show that expression levels of membrane-bound TNFR types I and II on different subpopulations of immunocompetent cells differ between patients with active pulmonary tuberculosis and healthy individuals and that these receptors are involved in the pathological process. %U https://erj.ersjournals.com/content/erj/42/Suppl_57/P2781.full.pdf