RT Journal Article SR Electronic T1 LSC 2012 abstract - Mesenchymal stromal cells identified in tissue from lung-transplanted patients JF European Respiratory Journal JO Eur Respir J FD European Respiratory Society SP 3102 VO 40 IS Suppl 56 A1 S. Rolandsson A1 A. Andersson-Sjöland A1 J. Brune A1 L. Eriksson A1 I. Skog A1 L. Hansson A1 L. Bjermer A1 S. Scheding A1 G. Westergren-Thorsson YR 2012 UL http://erj.ersjournals.com/content/40/Suppl_56/3102.abstract AB Background: Chronic rejection expressed as bronchiolitis obliterans (BO) is seen in 50% of lung-transplanted (LT) patients. BO is characterized by extra cellular matrix deposition, where the fibroblast is thought to be a key player. The fibroblast origin is at present not fully known. The fibrocyte is one of the potential origins, and our group has shown that fibrocytes are associated with remodelling in BO. In this study we focused on another origin the mesenchymal stromal cell (MSC).Objectives: Our aims were to examine whether MSC are present in tissue from LT patients. We wanted to evaluate whether these cells displays characteristic MSC properties such as adherent clonal growth, multi-lineage potential and a characteristic surface marker profile.Methods: MSC were isolated from biopsies of LT patients and the single cell suspension was seeded in colony-forming unit-fibroblast (CFU-F) assays. Isolated MSC were differentiated towards adipocytes, osteoblasts and chondrocytes.Their surface markers was examined by flow cytometry.Results: MSC isolated from LT patients formed typical colonies, displayed spindle-shaped morphology and adhered to plastic. Furthermore, cells displayed multi-lineage potential. Analysis of the surface markers showed that MSC showed expression of CD73, CD105 and CD90, but lacked expression of CD45 and CD34.Conclusions: Our study suggests that MSC are present in lung tissue from LT patients. These cells display colony growth, multipotential and a characteristic MSC surface marker profile.