PT  - JOURNAL ARTICLE
AU  - Nicolaj Denisevich
AU  - Ekaterina Kolesneva
AU  - Lyudmila Dubovskaya
AU  - Igor Volotovski
TI  - Serum biomarkers analysis in workers with occupational salt dust exposure in underground conditions in dependence of length of service
DP  - 2011 Sep 01
TA  - European Respiratory Journal
PG  - p4194
VI  - 38
IP  - Suppl 55
4099  - http://erj.ersjournals.com/content/38/Suppl_55/p4194.short
4100  - http://erj.ersjournals.com/content/38/Suppl_55/p4194.full
SO  - Eur Respir J2011 Sep 01; 38
AB  - Objective: We assessed serum biomarkers distribution in underground workers with occupational salt dust exposure in dependence of length of service.Methods: Serum samples of male donors who are not working at Belaruskaly, and workers employed in underground working conditions of Belaruskaly with a mean age 40±7 years were enrolled. All donors were divided into 4 groups.Group I included healthy donors who do not work in the enterprise; II - workers with underground work experience of 5 years; III- consisted of workers with underground work experience of 5 to 15 years; IV - workers with underground experience of more than 15 years.To analyze the qualitative composition of serum proteins were obtained by two-dimensional maps of proteins in blood serum from healthy donors and the study group by two-dimensional gel electrophoresis.Proteomic maps were statistically analyzed in dependence of length of service.Results: We found that total protein value in group II-IV differed from the samples of the 1st group. No statistically significant changes nave been revealed in the serum protein composition of underground workers.Proteomic maps revealed abnormalities indicating the presence of inflammation in the bronchopulmonary system of workers. This could be the diagnostic indicator of early stages of lung diseases in the absence of clinical evidence.Conclusions: Analysis of serum proteome changes under the influence of sylvinite aerosols could help to search for specific biomarkers of the body functioning of workers under the influence of occupational factors and predict risk of lung morbidity in occupational exposure to sylvinite aerosols.