RT Journal Article SR Electronic T1 Gene polymorphism of epidermal growth factor receptor (EGFR) and airway hyperresponsiveness (AHR) in young allergic subjects without respiratory symptoms JF European Respiratory Journal JO Eur Respir J FD European Respiratory Society SP p482 VO 38 IS Suppl 55 A1 Takahiro Yoshikawa A1 Hiroshi Kanazawa YR 2011 UL http://erj.ersjournals.com/content/38/Suppl_55/p482.abstract AB Background: Airway hyperresponsiveness (AHR) in young asymptomatic adults with atopy can be one of indicators of future symptomatic asthma. We aimed to examine genetic association of epidermal growth factor receptor (EGFR) with the asymptomatic AHR.Methods: We recruited one-hundred and eighty-nine allergic volunteers (mean age 19.5±1.4 y.o.) without history of respiratory symptoms, measured bronchial responsiveness to methacholine (0.3 to 10.0 mg/ml) and classified them into subjects with AHR and those without AHR according to a cutoff level of PC20 (8.0mg/ml). Then, we genotyped 12 SNPs on EGFR gene, and estimate total imbalance of haplotypes frequencies within haplotype block between two groups.Results: Seventy-two (38.1%) in 189 subjects exhibited 20% decrease in FEV1 from the baseline values by inhalation of methacholine 8.0mg/ml or less. Significant results were observed in the following haplotype block: rs 4947972 - rs 12718945 - rs 2072454 (block-1), rs 4947972 - rs 12718945 - rs 2227983 (block-2), or rs 4947972 - rs 12718945 - rs 2293347 (block-3). In each haplotype block, higher frequency of a haplotype, C-T-C (block-1), C-T-A (block-2) and C-T-A (block-3), was observed in subjects with AHR than in those without AHR (odds ratio and the corresponding P value; 3.289 and 0.00087 (block-1), 3.436 and 0.00055 (block-2), and 4.036 and 0.00181 (block-3), respectively). No significant difference was observed in serological parameters and pulmonary function between two subject groups.Conclusion: Our results indicate that the EGFR gene polymorphism might be associated with presence of asymptomatic AHR in young allergic adults.