RT Journal Article SR Electronic T1 Expression of osteopontin in the lung tissue of acute lung injury rats and the influence of osteopntin on the expression of TNF-a and IL-10 JF European Respiratory Journal JO Eur Respir J FD European Respiratory Society SP p2043 VO 38 IS Suppl 55 A1 Wenjun Wang A1 Xianming Fan YR 2011 UL http://erj.ersjournals.com/content/38/Suppl_55/p2043.abstract AB This work was supported by International cooperation and exchange program of Sichuan science and technology department (N0. 2009HH0032):Background: Pathogenesis of acute lung injury (ALI) has not been clarified fully, also there has no sure way to improve the prognosis of ALI.Aims: To investigate the expression of osteopontin (OPN) in the lung tissue of ALI rats and the influence of OPN on the level of TNF-a and IL-10 in serum.Methods: 56 SD rats were randomly divided into control group (24 rats), ALI group (24 rats)and intervention group (24 rats). 8 rats in each group were examined at 2, 4 and 8 h. ALI was induced by LPS injection via caudal vein in ALI and intervention groups, at the same time, rats in intervention group were also injected with anti-OPN antibody. Wet/Dry Weight (W/D), Pathological changes of the lung tissue and ALI score were detected. The level of TNF-a and IL-10 in serum and the expression of OPN in homogenate of lung tissue were detected.Results: W/D, ALI score and the level of TNF-a in ALI group at 2,4 and 8 h were higher than those in control group and intervention group at the same time point (p<0.01).The level of IL-10 in ALI group at 2,4 and 8 h was higher than that in control group (p<0.01), but was lower than that in intervention group at the same time point (p<0.05). Compared with control group, the expression of OPN in ALI and intervention groups at 2,4 and 8 h was higher (p<0.01).Conclusions: The expression of OPN in lung tissue of ALI rats induced by LPS was increased, and OPN could aggravate lung injury, which might be related to up-regulating expression of TNF-a and down-regulating expression of IL-10.