RT Journal Article
SR Electronic
T1 IP-10 is an additional marker to evaluate the RD1-specific responses in HIV-infected subjects
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP p4386
VO 38
IS Suppl 55
A1 Valentina Vanini
A1 Teresa Chiacchio
A1 Cristiana Gioia
A1 Gilda Cuzzi
A1 Nicoletta Orchi
A1 Alessia Rianda
A1 Lucia Alba
A1 Maria Letizia Giancola
A1 Aristide Conte
A1 Elisa Petruccioli
A1 Linda Petrone
A1 Enrico Girardi
A1 Delia Goletti
YR 2011
UL http://erj.ersjournals.com/content/38/Suppl_55/p4386.abstract
AB Background: The suboptimal sensitivity of IFN-γ-based assays, especially in immunocompromised individuals, emphasizes the need for alternative markers for diagnosing tuberculosis (TB). Objective of this study was to evaluate whether IP-10 can be a useful biomarker for evaluating a specific response to RD1 antigens associated to active TB in HIV-infected individuals. Control with QuantiFERON-TB Gold In tube (QFT-IT) was performed.Methodology: 118 HIV-infected individuals were prospectively enrolled in Rome, 21 with active-TB and 98 without. Epidemiological characteristics were analyzed. IFN-γ and IP-10 response to QFT-IT was performed. Plasma was harvested at day-1 and soluble factors evaluated by ELISA.Results: Significant differences between those with or without active TB were found for the CD4+ T cell counts (p=0.02), and IFN-γ and IP-10 response to QFT-IT (p=0.001 for both analysis). Differently no significant differences were found for the age and HIV-RNA. Based on the commercial cut-off of the QFT-IT and on a cut-off found by ROC analysis for the IP-10-based responses, the sensitivity for active TB of QFT-IT and the IP-10 to QFT-IT was 52% and 67% respectively (p=0.001; K: 0.545). The response to IP-10 was not influenced by the ability to respond to the mitogen. The specificity for active TB of QFT-IT and of the experimental test were 84% and 77% respectively (p=0.01; k:0.710). Among those without active TB a significant correlation between a positive score and Mtb exposure was found (p<0.001).Conclusions: These data suggest that IP-10 is an additional marker to evaluate the RD1-specific responses in HIV-subjects confirming data previously obtained in high TB endemic countries.