PT  - JOURNAL ARTICLE
AU  - Eri Hagiwara
AU  - Noriko Tsuchiya
AU  - Takahiro Enomoto
AU  - Tomohisa Baba
AU  - Takeshi Shinohara
AU  - Ryuichi Nishihira
AU  - Shigeru Komatsu
AU  - Terufumi Kato
AU  - Takashi Ogura
AU  - Hiroshi Takahashi
TI  - Antimicrobial resistance genotype trends and association with host clinical characteristics in respiratory isolates of <em>haemophilus influenzae</em>
DP  - 2011 Sep 01
TA  - European Respiratory Journal
PG  - p2555
VI  - 38
IP  - Suppl 55
4099  - http://erj.ersjournals.com/content/38/Suppl_55/p2555.short
4100  - http://erj.ersjournals.com/content/38/Suppl_55/p2555.full
SO  - Eur Respir J2011 Sep 01; 38
AB  - Objectives: We aimed to analyze eight-year trend of β-lactam resistance genotype in respiratory isolates of Haemophilus influenzae, and to clarify whether resistance genotypes were correlated with pathogenicity, virulence, and host clinical background.Methods: Respiratory isolates of H. influenzae from 2002 to 2009 in our hospital were classified into gBLNAS: β-lactamase TEM-1 negative (BLN) ampicillin susceptible with no resistant genes, gBLNAR: BLN ampicillin resistant with two PBP3 mutations, and other three genotypes using PCR. 144 strains isolated from different patients in 2008-09 were particularly analyzed for the association between genotypes and host clinical data.Results: Eight-year trend analysis showed the constant decrease of gBLNAS (43% to 30%) along with the steady increase of gBLNAR (15% to 53%). Among genotypes the possibility of being causative pathogen was the same in gBLNAR 51% and in gBLNAS 49%. There was no significant difference in the level of C-reactive protein and the white blood cell count in infectious diseases induced by gBLNAR and gBLNAS. Host clinical characteristics including age and gender were not different in gBLNAR and in gBLNAS except for underlying respiratory diseases. gBLNAR was found at the highest rate 92% in isolates from patients with non-tuberculous mycobacteriosis (n=11), followed by 61% from bronchiectasis and 55% from chronic bronchitis. In contrast, as low as 33% of isolates from COPD (n=18) were gBLNAR.Conclusions: There was no difference in pathogenicity and virulence between gBLNAR and gBLNAS in respiratory isolates. Underlying respiratory diseases impacted on the resistance genotype.