TY - JOUR T1 - Nitrosative and cytokine status in patients with COPD and chronic cerebrovascular diseases (CCVD) JF - European Respiratory Journal JO - Eur Respir J VL - 38 IS - Suppl 55 SP - p3894 AU - Svetlana Soodaeva AU - Timur Li AU - Igor Klimanov AU - Alexander Lisitsa AU - Nailja Kubysheva AU - Larisa Postnikova AU - Olga Mironova AU - Anatoliy Fedin Y1 - 2011/09/01 UR - http://erj.ersjournals.com/content/38/Suppl_55/p3894.abstract N2 - The aim of the study was to investigate the dynamics of nitric oxide (NO) stable metabolites concentration in exhaled breath condensate (EBC) as markers of the nitrosative stress and circulating inflammatory cytokines in COPD and CCVD.Material and methods: 50 males inpatients were enrolled in the study (age = 51-67 y.o.). All patients were divided in two groups: group1 contains 23 patients with COPD exacerbation and CCVD; group2 contains 27 patients with COPD exacerbation only. The control group consisted of 21 healthy volunteers.The investigation of NO metabolism was performed by the estimation of total nitrates/nitrites (TNN) level and 3-nitrothyrozine (3-NT) concentration both in EBC and in blood plasma. The TNN concentration was measured using the spectrophotometric method; 3-NT and cytokines (TNF-α, IL-8) concentrations in blood plasma were investigated with specific enzyme immunoassay.Results: The TNN levels in EBC as well as in blood plasma were significantly higher in group1&2 compared with control. The trend resulting to the increase of the TNN concentration in blood plasma was observed in patients with COPD and CCVD compared with COPD patients only (p=0.28). The 3-NT concentrations both in EBC and in blood plasma weren't changed in group1&2 compared with control. So, the statistically significant increase of inflammatory cytokines level in blood plasma compared with control was demonstrated (p<0,001). The cytokines activity in group1 was higher than in group2.Conclusion: The results obtained demonstrate the increase of nitrosative/cytokines stress parameters as a systemic reaction in patients with COPD and CCVD compared with COPD patients only. ER -