@article {Carusop4078, author = {Massimo Caruso and Giovanni Tringali and Nadia Pellegrino and Maria Proiti and Elisa Basile and Maria Cosentino and Riccardo Polosa}, title = {Basophil activation test as support for distinguishing between aspirin-induced asthma and allergic reaction to ASA}, volume = {38}, number = {Suppl 55}, elocation-id = {p4078}, year = {2011}, publisher = {European Respiratory Society}, abstract = {Aspirin-induced asthma (AIA),is a clinical syndrome associated with severe and chronic inflammation in both upper and lower airways.Despite their similarities,AIA is quite distinct from Acetyl Salicylic Acid (ASA) allergy.AIA remains largely underdiagnosed,mainly for the lack of diagnostic methods to detect ASA sensitisation without risk and to distinguish it from ASA allergy.Here we investigate if it is possible to apply a method designed for allergic sensitization,in order to distinguish hypersensitivity from allergy to ASA.This method is well-known as Basophil Activation Test (BAT),and it consist of in vitro blood cells stimulation by ASA and then detection of two activation markers (CD63/CD203c) on basophils,typical of allergic reaction.We performed BAT on peripheral blood samples from 8 subjects referring respiratory disorders after ASA assumption.The expression of markers were considered positive when the Stimulation Index (SI) was higher than 2,defining SI the ratio between markers on basophils stimulated and unstimulated.BAT evidenced activation in three subjects,indicating that they suffer from ASA allergy.The other five subjects did not show any basophil activation (BA).Since BA is a clear sign of allergic reaction,it is plausible to conclude that the subjects who do not respond to BAT are suffering from AIA,so we may assume that BAT is useful to distinguish between AIA and ASA allergy.}, issn = {0903-1936}, URL = {https://erj.ersjournals.com/content/38/Suppl_55/p4078}, eprint = {https://erj.ersjournals.com/content}, journal = {European Respiratory Journal} }