RT Journal Article
SR Electronic
T1 AQP5 role in ovalbumin-induced airway inflammation and secretion of MUC5AC in mice
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP p962
VO 38
IS Suppl 55
A1 Chunling Dong
A1 Guifang Wang
A1 Meiling Jin
A1 Bo Li
A1 Diane Wang
A1 Zhihong Chen
A1 Zhongsen Ma
A1 Xiangdong Wang
A1 Chunxue Bai
YR 2011
UL http://erj.ersjournals.com/content/38/Suppl_55/p962.abstract
AB Background: Airway inflammation and mucus hypersecretion are two important characteristic features of the pathogenesis of asthma. Aquaporin5 (AQP5) is known to be a water channel protein expressed widely in lung epithelium and submucosal glands.Objective: The present study aimed at investigating the involvement of AQP5 in asthma.Methods: The ovalbumin (OVA)-induced allergic pulmonary inflammation and MUC5AC production were examined in AQP5+/+ or AQP5–/– mice. The expression of AQPs in lung tissue and their regulation were detected. In addition, epidermal growth factor receptor (EGFR) expression and osmotic water permeability in lung were evaluated.Results: Lower expression of AQP1, 4, 5 while higher AQP3 was significant in lung tissue from AQP5+/+ model mice. Only AQP1 and AQP5 were up-regulated by anti-asthmatic agents (dexamethasone, ambroxol and terbutaline) significantly. However, AQP5 knockout had significantly low airway inflammation and less lung edema induced by OVA, as compared with those in AQP5+/+ mice. In addition, lower expression of MUC5AC in airway epithelium, less secretion of MUC5AC were found in AQP5–/– model mice. Moreover, the expression of EGFR on airway epithelium was prevented by AQP5 knockout in asthmatic model.Conclusion: Our data indicate that AQP5 is involved in the development of allergic airway inflammation and mucus hypersecretion by regulating osmotic water permeability and expression of EGFR.