RT Journal Article
SR Electronic
T1 Frequent detection of rhinovirus in bronchoalveolar lavage samples from children with cystic fibrosis
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP p4562
VO 38
IS Suppl 55
A1 Elisabeth Kieninger
A1 Caroline Tapparel
A1 M.-N. Kronig
A1 F. Singer
A1 P. Latzin
A1 Hui-Leng Tan
A1 Cara Bossley
A1 C. Casaulta
A1 Andrew Bush
A1 Jane C. Davies
A1 Laurent Kaiser
A1 Nicolas Regamey
YR 2011
UL http://erj.ersjournals.com/content/38/Suppl_55/p4562.abstract
AB Background: Rhinovirus (RV)-induced chest exacerbations are common in cystic fibrosis (CF) and have been associated with impaired virus clearance by the CF airway epithelium.Hypothesis: As a consequence RV can be frequently detected in lower airways of CF children.Patients and methods: Bronchoalveolar lavage (BAL) samples were collected from children with CF (n=93), non-CF bronchiectasis (n=26), asthma (n=19) and control children without lower respiratory tract disease (n=21) at a median (IQR) age of 6.7 (2.5-10.8) years. RV load was assessed by RT-PCR. Prevalence of RV infection and RV load were compared between groups and related to demographic and clinical parameters.Results: RV was detected in 58 samples (36% of total) and more often in younger children <5years of age (29 vs. 18%). RV prevalence was highest in CF (41%) compared to non-CF bronchiectasis (23%), asthmatic (32%) and healthy children (29%). In RV-positive subjects RV load (median [IQR] copies/ml) was highest in CF (300 [15–3400] ×103) compared to asthmatic (2.3 [2.1-4.8] ×103) (p=0.02), non-CF bronchiectasis (11 [3.5-193] ×103) (p=0.03) and healthy children (0.8 [0.4-6.5] ×103) (p=0.001). RV prevalence was similar in CF patients in whom BAL was performed during chest exacerbation (n=20) and phases of clinical stability (n=17). However, RV load was higher during exacerbation (859 [20-3380] ×103 vs. 25 [5.8-277] ×103), p=0.01) and inversely related to FEV1 (%predicted) (r=-0.52, p=0.004).Conclusions: RV is frequently detected in the lower airways of CF children. High RV loads during chest exacerbations and in children with advanced lung disease suggest a possible role for RV in CF lung disease progression.