RT Journal Article
SR Electronic
T1 TGFβ1 genotype in correlation to TGFβ1 induced sputum (IS) and serum in cystic fibrosis (CF)
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP p4552
VO 38
IS Suppl 55
A1 Sabina Schmitt-Grohé
A1 Felix Schreiner
A1 Louisa van den Boom
A1 Doris N'Gampolo
A1 Olaf Eickmeier
A1 Ralf Schubert
A1 Ilse Broeckart
A1 Stefan Zielen
A1 Michael Lentze
YR 2011
UL http://erj.ersjournals.com/content/38/Suppl_55/p4552.abstract
AB Background: Previous studies have shown that high-producer TGFβ1 genotypes are associated with severe lung disease in CF, but data on TGFβ1 levels and its impact on CF lung disease are scarce. Our aim was to assess the relationship between TGFβ1 genotypes, TGFβ1 (in induced sputum and serum) and lung disease.Methods: 23 patients delta F 508 homozygous (median age 24 y, m/f 13/10, BMI 20,96 kg/m2, Shwachman score 75, FEV1 (%) 84) were examined. TGFβ1 was assessed in serum and IS by ELISA. Genotyping was performed for the TGFβ1 C-509T and T+869C genotype.Results: TGFβ1 mutants (C-509T mutant/wildtype n=9/14; T+869C mutant/wildtype (7/16) had no influence on levels of TGFβ1 in IS (C-509T mutant/wildtype median 80.2/71.8 pg/ml, n.s; T+869C mutant/wildtype median 80.2/71.75 pg/ml, n.s) and serum (C-509T mutant/wildtype median 35.8/34.9 pg/ml, n.s; T+869C mutant/wildtype median 35.8/34.9 pg/ml, n.s) nor on leukocytes in IS (C-509T mutant/wildtype median 651/495/μl, n.s; T+869C mutant/wildtype median 651.25/495/μl, n.s) and in EDTA blood (C-509T mutant/wildtype median 6325/6990 pg/ml, n.s; T+869C mutant/wildtype median 6325/6990/μl, n.s), lung function resp FEV1 (%) (C-509T mutant/wildtype median 68.6/86.7%, n.s; T+869C mutant/wildtype median 60.3/86.66%, n.s) or BMI (C-509T mutant/wildtype median 21.9/19.2 pg/ml, n.s; T+869C mutant/wildtype median 22.41/19.52, n.s).Conclusion: Genotype had no difference on TGFβ1 in IS and serum as well as for lung function or BMI. An explanation is that TGF β is activated by different pathways e.g. ανβ6-mediated activation appears to be absolutely dependent on direct cell-cell contact and does not release any diffusible free TGF β (Munger et al 1999).