TY - JOUR T1 - Effects of <em>Bu-Zhong-Yi-Qi-Tang</em> (<em>Hochuekkito:</em> TJ-41) on the inflammatory responses in alveolar macrophages of hyperglycemic mice JF - European Respiratory Journal JO - Eur Respir J VL - 38 IS - Suppl 55 SP - p3865 AU - Masayuki Nakayama AU - Hideaki Yamasawa AU - Manabu Soda AU - Naoko Mato AU - Tatsuya Hosono AU - Masashi Bando AU - Yukihiko Sugiyama Y1 - 2011/09/01 UR - http://erj.ersjournals.com/content/38/Suppl_55/p3865.abstract N2 - This study examines the influence of Bu-Zhong-Yi-Qi-Tang (Hochuekkito; TJ-41) on the inflammatory response in alveolar macrophage in hyperglycemic mice. BALB/c mice (males, six to eight weeks old) were divided into three groups: A, B, and C. From the outset, group A and B were fed an ordinary diet, whereas TJ-41 was given to group C. After the initiation of these diets, intraperitoneal injection of streptozotocin (STZ), 250mg/g in mice of groups B and C. Four weeks after the start of these diets, blood-glucose levels were measured, and bronchoalveolar lavage (BAL) was performed. Alveolar macrophages were sampled, and those demonstrating blood-glucose levels of more than 200 mg/dL were selected for use. Toll-like receptor (TLR) ligands (TLR2: peptidoglycan [PGN], TLR4: LPS, TLR5: flagellin [FLG]) were employed to stimulate pulmonary-alveolar macrophages, and ELISA was used to measure TNF-a production. TLR expression on pulmonary-alveoli surface was evaluated on the basis of the emergence of each TLR mRNA in alveolar macrophages. In mice given STZ, stimulus with TLR2, 5 ligands significantly inhibited TNF-a production. But in mice given a diet including TJ-41 inhibition of TNF-a production improved significantly. No differences were observed in the expression of TLR mRNA in pulmonary-alveolar macrophages and on cell surfaces. These results suggests the possibility that TJ-41 may enhance inhibition of pulmonary-alveolar macrophage inflammation response to TLR-ligands stimulus in conditions of high blood glucose. ER -