RT Journal Article
SR Electronic
T1 Late-breaking abstract: Serum and blood cell culture Th17 cytokines in pigeon fanciers' hypersensitivity pneumonitis (HP)
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP p666
VO 38
IS Suppl 55
A1 Kenneth Anderson
A1 Iona Donnelly
A1 Lisa Jolly
A1 S. Todryk
A1 P. Pushparaj
A1 M. Lum
A1 G. Short
A1 M. Adamson
A1 Steven Bourke
A1 Charles McSharry
YR 2011
UL http://erj.ersjournals.com/content/38/Suppl_55/p666.abstract
AB While HP affects a proportion of subjects, the factors that determine susceptibility are poorly understood. In HP experimental models IL-17 has pro-inflammatory function coordinating the inflammatory cytokines however little is known about this system in humans in HP among pigeon fanciers.Aims: To quantify IL-1, IL-2, IL-4, IL-6, IL-10, IL-17, IL-22, TNFα, IFNγ, GM-CSF in serum and culture supernatant of immune blood cells of 45 pigeon fanciers and investigate their association with symptoms of HP.Methods: Cytokines in antigen-stimulated lymphocyte and monocyte culture assay and serum antibody were measured by enzyme immunoassay.Results: 11 subjects were categorised as HP probable, 6 were HP possible and 28 HP unlikely. The serum IgG antibody titre correlated with the number of symptoms, (r=0.288, p=0.048), and increased with the disease category of HP (r=0.397, p=0.007). There were no significant differences between clinical categories and plasma cytokine concentration or between the concentrations of serum IgG antibody and any of the cytokines, but there were trends for a negative correlation between the antibody with IL-10 (r= -0.309, p=0.053) and with IL-4 (r= -0.297, p=0.066). The serum antibody levels correlated with antigen-specific proliferation stimulation index (r=0.537, p=0.007), and with IL-2, IL-4 and IL-5 (p<0.05 for each). There was no disease specific serum or antigen-driven lymphocyte or monocyte culture production of IL-17 or IL-22.Conclusions: In this study of serum and peripheral blood cell cytokines, the evidence suggested a potential role for Th2 cytokines associated with HP. There was no evidence of Th17 lymphocyte subset involvement.