TY - JOUR T1 - The relationship of IL-4 cytokine gene polymorphisms, HRCT and histopathological score in patients with idiopathic pulmonary fibrosis JF - European Respiratory Journal JO - Eur Respir J VL - 38 IS - Suppl 55 SP - p1997 AU - Martina Vasakova AU - Martina Sterclova AU - Radoslav Matej AU - Libor Kolesar AU - Jelena Skibova AU - Ilja Striz Y1 - 2011/09/01 UR - http://erj.ersjournals.com/content/38/Suppl_55/p1997.abstract N2 - Introduction: Idiopathic pulmonary fibrosis (IPF) is a serious disease with unknown etiology, where an influence of cytokine gene polymorphisms is presumed. We compared HRCT alveolar (AS) and interstitial score (IS) and histoptahologic score with IL-4, and IL-4RA gene polymorphisms in IPF patients.Subjects and methods: IPF was diagnosed in 46 patients according to ATS/ERS consensus statement. 43 patients had evaluable HRCT investigations, 14 patients had surgical lung biopsy. HRCT scans were evaluated using IS and AS scales by Gay et al. The histopathological evaluation of lung biopsies comprised: myofibroblast foci (MF), inflammation, eosinophils, granulomas and Ashcroft criteria for fibrosis grading. The IL-4 (-1098) (-590) (-33) and IL-4 RA +1902 gene polymorphisms were characterized utilizing a PCR- SSP method.Results: AS was higher in IL-4 haplotypes 1 TTC and TTT carrierrs (p=0.0423). Ashcroft score was more advanced in IL-4 haplotype 2 GCC (p= 0,013) and MF counts were higher in TCC carriers (p=0,0736). IL-4 RA +1902 A1 G and IL-4 -590 A1 T correlated with higher AS (p=0,0335; p=0,0123). Ashcroft score was higher in IL-4 -1098 A2 G and IL-4 -33 A1 T carriers (p= 0,0443, p=0,0915).Conclusions: We assume that IL-4 and IL-4RA polymorphisms might influence HRCT and histopathological phenotype of IPF. The correlation of functionally relevant IL-4 genes polymorphisms (especially IL-4 -33 T) with AS could mean, that new alveolar lesions with continuing fibrosis are more pronounced in these polymorphisms carriers. The positive correlation of IL-4 -33 A1 T with Ashcroft score might support a hypothesis of fibrogenetic role of IL-4 in IPF. ER -