PT  - JOURNAL ARTICLE
AU  - Rui Zhou
AU  - Yi He
AU  - Ping Chen
AU  - Guang-Sen Zhang
AU  - Shi-Hai Zou
TI  - Adenovirus-mediated AP-2α down-regulates MnSOD expression in lung cancer A549 cells and its molecular mechanism
DP  - 2011 Sep 01
TA  - European Respiratory Journal
PG  - p1487
VI  - 38
IP  - Suppl 55
4099  - http://erj.ersjournals.com/content/38/Suppl_55/p1487.short
4100  - http://erj.ersjournals.com/content/38/Suppl_55/p1487.full
SO  - Eur Respir J2011 Sep 01; 38
AB  - Objective: MnSOD is potential therapy target for lung cancer and it is important to control MnSOD aberrant expression in lung cancer cells. In this study, we investigate the effect of AP-2α activation on MnSOD expression in lung cancer cell line and provide experimental evidence on the applications of AP-2 for lung cancer therapy.Methods: A549 cells were transfected with the Adenovirus-AP-2α construct (Ad-AP-2α) at multiplicities of infection (MOI) of 0, 10, 30, 50 MOI or with the adenovirus-lacZ reporter gene construct (Ad-lacZ) at 50 MOI. A549 cells were transfected for 0, 6, 12, 24, 48 hrs with Ad-AP-2α at 50 MOI and also were transfected for 24hrs with the dominant-negative mutant AP-2α construct with liposome-mediated method. The time- and dose-dependent effects of AP-2 on MnSOD expression were detected by RT-PCR and Western blot.Results: (1) With increasing Ad-AP-2 titers, the expression of MnSOD is decreased. (2) Transfected with 50 MOI Ad-AP-2α and measured expression of MnSOD at 0, 6, 12, 24 and 48 hr, we observed a concomitant decreasing in MnSOD expression. (3) Compared with the controls, the expression of MnSOD was declined after transferring the dominant-negative mutant AP-2α construct into lung cancer (A549) cells.Conclusions: The results suggest that AP-2 represses MnSOD expression in lung cancer cells and down-regulation of MnSOD may be related to DNA binding domain of AP-2α.