RT Journal Article
SR Electronic
T1 Amorfous components of the extracellular matrix have impact as new biochemical markers on malignancy characterization and prognosis of non-small cell lung cancer
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP 4503
VO 38
IS Suppl 55
A1 Maristela Peres Rangel
A1 Vanessa Karen de Sá
A1 João Roberto Maciel Martins
A1 Edwin Roger Parra
A1 Aline Mendes
A1 Dirce Carrasco
A1 Eloísa Olivieri
A1 Vera Luiza Capelozzi
YR 2011
UL http://erj.ersjournals.com/content/38/Suppl_55/4503.abstract
AB Introduction: Many have reported that glycosaminoglycans (GAGs) have different behaviors in the presence of malignant tissues.In this study, we examined different GAGs concentrations and their impact on diagnosis and/or prognosis of patients with non small cell lung cancer.Methods: Sulfated GAGs and Hyaluronic Acid (HA) were examined in tumoral and non-tumoral tissues from 45 lung cancer patients.Tissue samples were dehydrated and incubated with a proteolytic enzyme.The levels of HA were measured by a noncompetitive ELISA-like fluorometric assay. The sulfated GAG chains (heparan, dermatan and chondroitin sulfate - HS, DS and CS), were precipitated, dissolved in DNAse and their concentrations were identified after gel electrophoresis.Results: HS and HA showed significantly higher concentration in tumoral than in normal areas (p=0.02 and p=0.0001, respectively; Fig. 1A/B). The Kaplan-Meier survival curves shows that tissues with lower concentrations of HA have better long-term survival than those with higher concentrations (Log Rank=3,59; p=0.05; Fig. 1C). One hundred% of tumoral areas presented CS while the normal almost never (p=0.0001; Fig. 1D).Conclusions: The results presented suggest a possible role of these molecules on lung cancer development, but more importantly provide potential biochemical markers for differentiating normal from lung cancer patients.