@article {Artaud-Macari175, author = {Elise Artaud-Macari and Delphine Goven and St{\'e}phanie Brayer and Joelle Marchal-Somme and Bruno Crestani and Anne Boutten and Marcel Bonay}, title = {Antifibrosic effects of sulforaphane in human lung fibroblasts from idiopathic pulmonary fibrosis}, volume = {38}, number = {Suppl 55}, elocation-id = {175}, year = {2011}, publisher = {European Respiratory Society}, abstract = {Rationale: Nrf2 pathway has been implicated in myofibroblastic differentiation, a key step in idiopathic pulmonary fibrosis (IPF) pathophysiology. Sulforaphane (SFN), an isothiocyanate compound found in cruciferous vegetables, is known to activate Nrf2. The aim of this study was to assess the effects of SFN on oxidative stress and fibroblast phenotype in IPF.Methods: The effects of SFN were assessed on human pulmonary fibroblasts from IPF and control patients in vitro. Oxidant/antioxidant balance, nuclear Nrf2 expression, fibroblast phenotype, in basal and profibrosing conditions (TGF-β or PDGF-BB stimulation) were experienced.Results: SFN increased antioxidant enzymes and Nrf2 nuclear expression, and decreased oxidative stress in IPF fibroblasts, in basal conditions and after TGF-β stimulation. SFN stimulation induced a myofibroblastic dedifferentiation of IPF fibroblasts with morphologic aspect of control-like fibroblasts and concomitant reduction of α-SMA and collagen I expression, proliferation, migration and contraction. Moreover, addition of SFN after TGF-β and PDGF-BB stimulation, inhibited their deleterious effects on IPF and control fibroblasts, and restored antioxidant defenses. Nrf2 siRNA transfection abolished antifibrosing effects of SFN.Discussion: The effects of SFN on cell differentiation have been reported in renal tubular epithelial cells in a rat model of renal fibrosis, but its effects on human pulmonary fibroblasts from IPF and control patients were unknown.Conclusion: These results suggest the potential therapeutic effect of SFN on pulmonary fibroblasts in IPF in vitro.}, issn = {0903-1936}, URL = {https://erj.ersjournals.com/content/38/Suppl_55/175}, eprint = {https://erj.ersjournals.com/content}, journal = {European Respiratory Journal} }