TY - JOUR T1 - Altered phenotype of blood dendritic cells in patients with acute pneumonia JF - European Respiratory Journal JO - Eur Respir J VL - 38 IS - Suppl 55 SP - p3873 AU - Katharina Dreschler AU - Kai Bratke AU - Sebastian Petermann AU - Petra Thamm AU - Michael Kuepper AU - J. Christian Virchow AU - Marek Lommatzsch Y1 - 2011/09/01 UR - http://erj.ersjournals.com/content/38/Suppl_55/p3873.abstract N2 - Background: Dendritic cells (DCs) play a key role in the host defense against inhaled pathogens. However, the phenotype of blood DCs in patients with acute respiratory infections is unknown.Objective: To investigate the number and the expression of function-associated molecules of blood DCs in patients with acute infectious pneumonia.Methods: Sixteen patients with an acute pneumonia and 19 controls without pneumonia were included in the study. The number as well as the expression of function-associated molecules of myeloid DCs (mDCs) and plasmacytoid DCs (pDCs) were analysed in peripheral blood using four-colour flow cytometry.Results: Elevated concentrations of procalcitonin (median: 0.55 ng/ml) and the rapid response to antibiotic treatment suggested a bacterial origin of the pneumonia in the patients. Total mDC (median: 27% of the controls) and pDC counts (median: 53% of the controls) were markedly reduced in patients with pneumonia, as compared to controls. Percentages of blood mDCs, but not pDCs, were negatively correlated with serum concentrations of c-reactive protein (CRP). Patients with pneumonia were characterised by a significantly increased expression of Fc gamma receptors (CD32 and CD64) on mDCs and the Toll-like receptor 9 (TLR9) in pDCs.Conclusions: Circulating DCs are markedly reduced in patients with pneumonia, and characterised by an upregulation of molecules recognising pathogen-associated molecular patterns (PAMPs) and opsonised antigens. ER -