%0 Journal Article %A Katharina Dreschler %A Kai Bratke %A Sebastian Petermann %A Petra Thamm %A Michael Kuepper %A J. Christian Virchow %A Marek Lommatzsch %T Altered phenotype of blood dendritic cells in patients with acute pneumonia %D 2011 %J European Respiratory Journal %P p3873 %V 38 %N Suppl 55 %X Background: Dendritic cells (DCs) play a key role in the host defense against inhaled pathogens. However, the phenotype of blood DCs in patients with acute respiratory infections is unknown.Objective: To investigate the number and the expression of function-associated molecules of blood DCs in patients with acute infectious pneumonia.Methods: Sixteen patients with an acute pneumonia and 19 controls without pneumonia were included in the study. The number as well as the expression of function-associated molecules of myeloid DCs (mDCs) and plasmacytoid DCs (pDCs) were analysed in peripheral blood using four-colour flow cytometry.Results: Elevated concentrations of procalcitonin (median: 0.55 ng/ml) and the rapid response to antibiotic treatment suggested a bacterial origin of the pneumonia in the patients. Total mDC (median: 27% of the controls) and pDC counts (median: 53% of the controls) were markedly reduced in patients with pneumonia, as compared to controls. Percentages of blood mDCs, but not pDCs, were negatively correlated with serum concentrations of c-reactive protein (CRP). Patients with pneumonia were characterised by a significantly increased expression of Fc gamma receptors (CD32 and CD64) on mDCs and the Toll-like receptor 9 (TLR9) in pDCs.Conclusions: Circulating DCs are markedly reduced in patients with pneumonia, and characterised by an upregulation of molecules recognising pathogen-associated molecular patterns (PAMPs) and opsonised antigens. %U