TY - JOUR T1 - Influence of AECOPD to ROS production in neutrophils JF - European Respiratory Journal JO - Eur Respir J VL - 38 IS - Suppl 55 SP - p736 AU - Mindaugas Vaitkus AU - Simona Lavinskiene AU - Kristina Bieksiene AU - Jolanta Jeroch AU - Raimundas Sakalauskas Y1 - 2011/09/01 UR - http://erj.ersjournals.com/content/38/Suppl_55/p736.abstract N2 - Introduction and background: Chronic obstructive pulmonary disease (COPD) is characterized by specific pattern of inflammation involving polymorphonuclear neutrophils (PMN), macrophages and other cells. PMN are the major reactive oxygen species (ROS) producing cells. Our previous data showed that ROS production is decreased in stable COPD (SCOPD). It is still not known about ROS production in acute exacerbation of COPD (AECOPD).Aim: To investigate ROS production in PMN during AECOPD.Methods: 10 patients with SCOPD, 5 with AECOPD and 10 non-smoking healthy individuals (HI) were studied. PMN were isolated from peripheral blood using a high-density gradient. ROS production was induced using phorbol-myriste-acetate (PMA, 0.1-30 nM) and S. aureus bacteria (1-167 bacteria/neutrophil). ROS production was measured flow cytometrycally by mean of fluorescence intensity in PMN population.Results: The production of spontaneous ROS in PMN was 29.7±4.1% higher in AECOPD group than in SCOPD (p<0.05). PMA stimulated ROS production in all groups. The most significant increase of ROS production was observed between 0.3 and 1 nM PMA (AECOPD 172.5-fold, SCOPD 90.1-fold, HI 46.2-fold (p<0.05)).The higher ROS production in PMN after stimulation with different S. aureus concentration was found in AECOPD group compared with SCOPD and HI (p<0.05).Conclusion: Our data shows that ROS production is increased in AECOPD. Chemical (PMA) and biological (S. aureus bacteria) factors activates more intensive ROS generation. ER -