TY - JOUR T1 - Apoptosis of peripheral blood lymphocytes in different periods of asthma JF - European Respiratory Journal JO - Eur Respir J VL - 38 IS - Suppl 55 SP - p1841 AU - Zhanna Antanovich AU - Vladimir Tsarev AU - Nataliya Goncharova Y1 - 2011/09/01 UR - http://erj.ersjournals.com/content/38/Suppl_55/p1841.abstract N2 - Background: Resistance of lymphocytes to apoptosis can cause decrease of these cells elimination from respiratory ways and play a key role in persistence of inflammation in asthma.Objective: The aim of this study was to reveal the disturbances of blood lymphocytes apoptosis in different periods of asthma.Methods: 25 steroid naïve asthmatic adults with asthma exacerbation and 25 with asthma remission were randomly selected and 20 matched control subjects were included. All were submitted to detailed clinical history and examination, pulmonary function testing. Peripheral blood lymphocytes (PBLs) were stimulated by phytoagglutinin (PHA) and cultured for 72 hours. Apoptosis of PBLs was determined by flow cytometry by evaluating of Annexin V expression for detection of early apoptotic cells (Annexin V positive-PI negative).Results: Subjects with asthma exacerbation had significantly lower values of early apoptotic cells in blood than subjects with asthma remission (20,29±1,69% vs 41,65±2,38%; p<0,001) and controls (20,29±2,38% vs 43,02±2,58%; p<0,001). But there were no differences in values of early apoptotic cells in subjects with asthma remission and controls (41,65±11,91% vs 43,02±11,54%; p>0,05).Conclusion: The value of early apoptotic cells in 72 hours cultures of stimulated by PHA lymphocytes is a sensitive marker for asthma exacerbation and remission and can be predictor for asthma exacerbation. ER -