PT  - JOURNAL ARTICLE
AU  - Gunther Becher
AU  - Anne Hillmann
AU  - Roman Purkhart
AU  - Martina Graupner
AU  - Rolf Graupner
TI  - Adaptation of differential ion mobility spectrometry (DMS) for discrimination of specific biomarkers in exhaled breath in patients with severe renal-pulmonary dysfunction
DP  - 2011 Sep 01
TA  - European Respiratory Journal
PG  - p4041
VI  - 38
IP  - Suppl 55
4099  - http://erj.ersjournals.com/content/38/Suppl_55/p4041.short
4100  - http://erj.ersjournals.com/content/38/Suppl_55/p4041.full
SO  - Eur Respir J2011 Sep 01; 38
AB  - Introduction: Volatile substances (VOC's) in exhaled breath are target for identification of new biomarkers for disease and metabolic processes. Renal insufficiency could be a good example of an illness with exhaled markers of an internal disease.The Differential Ion Mobility Spectrometry (DMS) is a method for detection of volatile compounds in exhaled air with a sensitivity in ppt-range. The method was used in a pilot study for discrimination of patients with chronic metabolic kidney diseases.Methods: A DMS of SIONEX was used for analysis of exhaled breath. The measurements were performed before and after a dialysis procedure. The DMs-analysis includes a pre separation by a multi-capillary tube, ionization of the sample and measurement of ions by IMS with 270 sec. Spectra were discriminated by detection of clusters and calculation of significance using support vector machine.Results: It was possible to collect sufficient samples in all patients. Specific clusters of biomarkers were found discriminating marker exhalation before and after therapeutic intervention with dialysis. Specific clusters, indicating drug-uptake, were found.The inter-individual reproducibility was very high, which possibly represents the manifold drug-treatment and severity of renal insufficiency with enhanced blood urea.Discussion: Characteristic breath pattern could be detected. The method is non-invasive and fast and could offer new possibilities for long term control of medicaments and chronic metabolic disorders. Further studies are needed to identify certain markers and metabolites.