TY - JOUR T1 - The dynamics of free radical parameters in exhaled breath condensate (EBC) in smokers during the course of physical workload JF - European Respiratory Journal JO - Eur Respir J VL - 38 IS - Suppl 55 SP - p1800 AU - Elena Zaprudnova AU - Natalia Popova AU - Svetlana Soodaeva AU - Igor Klimanov AU - Alexander Lisitsa AU - Timur Li Y1 - 2011/09/01 UR - http://erj.ersjournals.com/content/38/Suppl_55/p1800.abstract N2 - The aim of the study was to investigate the total nitrates/nitrites (TNN) and Fe2+ concentrations in EBC in smokers compared with nonsmokers during the course of physical exercises.Materials and methods: The study group included 30 smokers 20-23 y.o. with smoking history of 3-4 years. The control group consisted of 25 healthy (without any documented medical diagnosis) non-smokers 20-23 y.o. The TNN level in EBC was determined by nitrate reductor and Griess reagent, the concentration of Fe2+ was observed by ferrozin method. All the data were determined in two points: baseline at rest and after exercise (when the heart rate was 100-120 beats per minute) in both groups.Results: The results obtained demonstrate that there was significant decrease of TNN concentration in EBC in study group (the basal level of TNN in EBC was lower in 1.6 times compared with nonsmokers). During the course of physical exercises it was shown the increase of TNN level in EBC in smokers compared with nonsmokers (in 2.0 and 1.2 times respectively), also the increase of Fe2+ basal concentration in lung tissue in 2 times compared with nonsmokers (p <0.05) was determined. During the exercise the Fe2+ concentration in smokers was higher in 1.4 times as compared with nonsmokers (p <0.05).Conclusion: The physical workload provide a significantly changes in free radical metabolism in smokers ER -