PT  - JOURNAL ARTICLE
AU  - Elena Payá Peñalver
AU  - Carmen Soto Fernández
AU  - Rubén Andújar Espinosa
AU  - Consuelo Alcalde Rumayor
AU  - Julia Guardiola Martínez
AU  - Juan Orlando Lόpez Ojeda
AU  - Carlos Federico Άlvarez Miranda
AU  - Francisco José Ruiz Lόpez
AU  - Manuel Lorenzo Cruz
TI  - Relationship between % neutrophils in induced sputum and IL-6 and TNF-α in COPD
DP  - 2011 Sep 01
TA  - European Respiratory Journal
PG  - p727
VI  - 38
IP  - Suppl 55
4099  - http://erj.ersjournals.com/content/38/Suppl_55/p727.short
4100  - http://erj.ersjournals.com/content/38/Suppl_55/p727.full
SO  - Eur Respir J2011 Sep 01; 38
AB  - Introduction: The percentage of sputum neutrophils are increased in patients by Chronic Obstructive Pulmonary Disease (COPD), and is a biomarker of airway inflammation. The aim of this study is the relationship between sputum% neutrophils and levels of IL-6 and TNFα in induced sputum in COPD with moderate-severe airflow obstruction.Method: Sputum samples were obtained using 3% saline, according to international guidelines., from a total of 60 subjects 40 COPD patients (criteria for diagnostic and classification of disease severity based on the Global Initiative for Chronic Obstructive lung disease (GOLD) and 20 healthy men (control group) who had no story of lung disease and normal spirometry. All the patients were smokers of >20 pack/year, and were free from acute exacerbations in three months prior to the study. In each subject BMI (kg/m2), dyspnoea according to the modified Medical Research Council scale (MRC), oxygen saturation (SatO2), spirometry before and after 400μg inhaled salbutamol, lung volumes and carbon monoxide transfer factor coefficient (KCO) measurements were measured. TNF-α and IL6 were meassured by RIA.Results: The sputum neutrophil% was statistic significative higher in COPD patients than group control p<0,05. We find a negative correlation between the percentage of the non-scamous cell count and% neutrophil whit the FEV1% and the% FEV1/FVC p< 0,05, but not with the grade of dyspnea (MRC), KCO, and SatO2.There was a weak association between sputum neutrophils and Il-6 r= 0,385, p<0,003, and TNF r= 0,468 p= 0,001Conclusion: Neutrophils in sputum in COPD patients are associated with the airflow limitation and whit the levels of IL-6 and TNF-α.Proyect FFIS/EMER09/18