%0 Journal Article %A Catherine Moermans %A Donat Degroote %A Pierre Dorfman %A Etienne Capieaux %A Monique Henket %A Jean-Louis Corhay %A Renaud Louis %T Late-breaking abstract: Differential expression profiles of genes involved in oxidative stress and inflammation in blood and sputum from healthy subjects and COPD patients %D 2011 %J European Respiratory Journal %P p762 %V 38 %N Suppl 55 %X Background: Environmental (mainly cigarette smoke) and genetic factors are known to be involved in the development of chronic obstructive pulmonary disease (COPD); however, a better understanding of the COPD genes expression dysregulation remains a major challenge.Increased oxidative stress is thought to be central in COPD pathogenesis and directly involved in local and systemic inflammation.Methods: We have investigated, by RT-PCR array, the mRNA expression profile of 95 genes involved both in inflammation and oxidative stress in sputum and blood from COPD patients (n = 18) and healthy controls (n = 17). We have used Ingenuity Pathway Analysis Software (IPA) to identify the networks of interactions, the biological processes and pathways in which genes showing a significant expression modification are involved.Results: In the blood cells of COPD, around half of genes showed modifications (26 up- and 19 down-regulated) compared to healthy controls and these were essentially involved in inflammation. Using IPA, we found that the most important cellular function altered was the cellular movement. In sputum cells, only 13 genes showed modifications (6 up- and 7 down-regulated, five were common with blood), most of them being involved in free radical scavenging and cell death.Conclusions: Compared to healthy subjects, there was a clear dysregulation in gene expression at systemic level, and to a lesser extent, at airway level. Therefore, gene expression profile shows differences between local and systemic compartments. %U