RT Journal Article
SR Electronic
T1 The application of mass spectrometry to the analysis and characterization of protein and peptide composition in exhaled breath condensate of pneumo-oncological diseased persons
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP p4034
VO 38
IS Suppl 55
A1 Guzel Kireeva
A1 Anna Ryabokon
A1 Aleksey Kononikhin
A1 Vladimir Bagrov
A1 Oleg Pikin
A1 Evgenij Nikolaev
A1 Eldar Anaev
A1 Sergey Varfolomeev
YR 2011
UL http://erj.ersjournals.com/content/38/Suppl_55/p4034.abstract
AB In recent years exhaled breath condensate (EBC)has been investigated more and more extensively as a matrix that reflects the composition of the airway-lining fluid and may contain biomarkers of diseases of respiratory system.The aim of this study is to compare identify proteins and peptides in EBC samples collected from two group of people with healthy pulmonary system and with verified oncological diseases patients of pneumonic system from Moscow Scientific Research Oncology Institute with using mass spectrometry, as well as to compare proteome identification.Bathes were collected using R-Tube, freeze dried, treated by trypsin and analyzed by nanoflow LC-MS/MS with a 7-Tesla Finnigan LTQ-FT mass spectrometer (Thermo Electron, Germany), by means of Bioworks Browser 3.1 SR1 (Thermo Electron, Germany) were generated list of direct peptide mass and mass of their fragments, with following identification of proteins in the databases thought Mascot (Matrix Science version 2.0.04, the UK). At collected samples were identified peptides from more that 40 proteins of different nature, e.g. keratins and non- keratins. Peptides of nuclear ubiquitous casein and cyclin-dependent kinases substrate (NUCKS) which located in the nuclei in proliferating cells, glutamine synthetase (GS)-an enzyme that plays an essential role in the metabolism of nitrogen, alpha 1-Antitrypsin (A1AT) is a protease inhibitor belonging to the serpin superfamily were discovered in disease EBC samples. They are uncharacteristic of healthy EBC samples. In conclusion, each of abnormal peptides, as well as their combinations, may have diagnostic value.