@article {Kireevap4034, author = {Guzel Kireeva and Anna Ryabokon and Aleksey Kononikhin and Vladimir Bagrov and Oleg Pikin and Evgenij Nikolaev and Eldar Anaev and Sergey Varfolomeev}, title = {The application of mass spectrometry to the analysis and characterization of protein and peptide composition in exhaled breath condensate of pneumo-oncological diseased persons}, volume = {38}, number = {Suppl 55}, elocation-id = {p4034}, year = {2011}, publisher = {European Respiratory Society}, abstract = {In recent years exhaled breath condensate (EBC)has been investigated more and more extensively as a matrix that reflects the composition of the airway-lining fluid and may contain biomarkers of diseases of respiratory system.The aim of this study is to compare identify proteins and peptides in EBC samples collected from two group of people with healthy pulmonary system and with verified oncological diseases patients of pneumonic system from Moscow Scientific Research Oncology Institute with using mass spectrometry, as well as to compare proteome identification.Bathes were collected using R-Tube, freeze dried, treated by trypsin and analyzed by nanoflow LC-MS/MS with a 7-Tesla Finnigan LTQ-FT mass spectrometer (Thermo Electron, Germany), by means of Bioworks Browser 3.1 SR1 (Thermo Electron, Germany) were generated list of direct peptide mass and mass of their fragments, with following identification of proteins in the databases thought Mascot (Matrix Science version 2.0.04, the UK). At collected samples were identified peptides from more that 40 proteins of different nature, e.g. keratins and non- keratins. Peptides of nuclear ubiquitous casein and cyclin-dependent kinases substrate (NUCKS) which located in the nuclei in proliferating cells, glutamine synthetase (GS)-an enzyme that plays an essential role in the metabolism of nitrogen, alpha 1-Antitrypsin (A1AT) is a protease inhibitor belonging to the serpin superfamily were discovered in disease EBC samples. They are uncharacteristic of healthy EBC samples. In conclusion, each of abnormal peptides, as well as their combinations, may have diagnostic value.}, issn = {0903-1936}, URL = {https://erj.ersjournals.com/content/38/Suppl_55/p4034}, eprint = {https://erj.ersjournals.com/content}, journal = {European Respiratory Journal} }