PT  - JOURNAL ARTICLE
AU  - Paolo Montuschi
AU  - Debora Paris
AU  - Dominique Melck
AU  - Vincenzina Lucidi
AU  - Giovanni Ciabattoni
AU  - Andrea Motta
TI  - Methodological aspects of nuclear magnetic resonance spectroscopy of exhaled breath condensate
DP  - 2011 Sep 01
TA  - European Respiratory Journal
PG  - p4044
VI  - 38
IP  - Suppl 55
4099  - http://erj.ersjournals.com/content/38/Suppl_55/p4044.short
4100  - http://erj.ersjournals.com/content/38/Suppl_55/p4044.full
SO  - Eur Respir J2011 Sep 01; 38
AB  - We investigated the following methodological aspects of nuclear magnetic resonance (NMR) spectroscopy-based metabolomics of exhaled breath condensate (EBC): 1) within-day, between-day and technical repeatability; 2) detection limit; 3) possible EBC contamination with saliva or cleaning solution for disinfection of EBC collection setup. A 600 MHz Bruker Avance spectrometer (Bruker BioSpin, Rheinstetten, Germany) was used. EBC was collected with a condenser (Ecoscreen, Jaeger, Hoechberg, Germany). Within-day repeatability of NMR spectroscopy, expressed according to Bland-Altman, was assessed in 5 healthy and 11 cystic fibrosis subjects collecting separate samples twice within the same day. All values were within the 2SD range. Between-day repeatability was assessed in the same subjects collecting 3 samples on day 1,3,7. Technical repeatability was assessed measuring 3 different samples 10 times. Between-day and technical repeatability were expressed as intraclass correlation coefficient which was 0.93 and 0.96, respectively. Detection limit was 0.14 μM. EBC and salivary metabolomic spectra were different as signals in 3.3-4.5 ppm region in saliva were absent in EBC. Apart from reference TSP, no peak was observed in the cleaning solution (sodium hypochlorite, 3.55 mM) spectrum.This technique has a low detection limit for NMR spectroscopy, is reproducible and not affected by contamination with saliva or cleaning solution.