TY - JOUR T1 - Immunocytochemical study of interleukin-1β in alveolar macrophages of patients with well-controlled and non-controlled asthma JF - European Respiratory Journal JO - Eur Respir J VL - 38 IS - Suppl 55 SP - p3844 AU - Svetlana Pospelova AU - Vyacheslav Kravtsov AU - Svetlana Sobchenko AU - Alexander Parfionov Y1 - 2011/09/01 UR - http://erj.ersjournals.com/content/38/Suppl_55/p3844.abstract N2 - We have conducted dynamic immunocytochemical investigation of the level of Interleukin-1β (IL-1β) in alveolar macrophages of a group of patients (n=16) with well-controlled and non-controlled asthma. Induced sputum specimens from each of the patients were collected twice. Cytologic preparations of sputum were obtained via method of liquid cytology. Cytologic centriphugates were coloured on slides via immunocytochemical method with the use of monoclonal anti-IL-1β antibodies. The results of immunocytochemistry were evaluated using Histology Score method (HScore).The average value of the investigated index “content of IL-1β in alveolar macrophages” in patients with non-controlled asthma was 251,5±8,3 HScore, while in patients with well controlled asthma it came to 66,4±18,0 HScore. The level of IL-1β decreased more than five times in 14 of 16 patients as a result of their treatment which included education.Thus, the results of our study indicate that IL-1β is produced by alveolar macrophages in significant amount during non-controlled asthma and that its production decreases abruptly when asthma becomes well-controlled. ER -