TY - JOUR T1 - Cigarette smoke exposure in mice leads to a loss of reversible cysteine oxidations PSSG and PSNO in lung JF - European Respiratory Journal JO - Eur Respir J VL - 38 IS - Suppl 55 SP - p3829 AU - Ine Kuipers AU - Ken Bracke AU - Guy Brusselle AU - Emiel Wouters AU - Niki Reynaert Y1 - 2011/09/01 UR - http://erj.ersjournals.com/content/38/Suppl_55/p3829.abstract N2 - It is established that cigarette smoke causes irreversible oxidations in lung tissue. However, its impact on reversible and physiologically relevant redox-dependent protein modifications remains to be investigated. In this study the effect of cigarette smoke exposure in vivo was investigated on the covalent binding of glutathione to protein thiols, known as S-glutathionylation (PSSG), which can be reversed by the enzyme glutaredoxin 1 (Grx1). Also, protein S-nitrosylation (PSNO) which is the modification of protein thiols by NO and which is reversed by the enzyme ADH5 was investigated.Both PSSG and PSNO levels in lung tissue were markedly attenuated after four weeks of cigarette smoke exposure in mice. This coincided with an attenuation of protein free thiol levels and an increase in protein carbonyl levels. Grx1 mRNA expression and activity were attenuated as well, whereas no alterations in expression or activity of ADH5 were observed.Taken together, cigarette smoke exposure decreases reversible cysteine oxidations PSSG and PSNO, which does not result in an increase in free thiols. These alterations are likely not the result of differences in regulatory enzymes, but of oxidative stress. ER -