TY - JOUR T1 - Genetic polymorphism of alpha 1 antytrypsin and glutathione S transferase and lung cancer risk JF - European Respiratory Journal JO - Eur Respir J VL - 38 IS - Suppl 55 SP - p1966 AU - Andreea Catana AU - Radu Popp AU - Monica Pop AU - Mihai Porojan AU - Victor Pop AU - Adrian Trifa AU - Felicia Petrisor AU - Marius Farcas Y1 - 2011/09/01 UR - http://erj.ersjournals.com/content/38/Suppl_55/p1966.abstract N2 - Background: Polymorphisms for genes encoding alpha 1 antitrypsin (A1AT) and glutathione S-transferase (GSTM1/GSTT1) might contribute to the variability in individual susceptibility to lung cancer.Objectives: This is a cross-sectional, randomized, case control study for the evaluation of the frequency of A1AT (MS, MZ) and GST (GSTM1/GSTT1 null) alleles among patients with lung cancer. The study included 56 cases of lung cancer diagnosed patients (histopathological examination), recruted from the Pneumology Hospital Leon Daniello Cluj and 125 healthy unrelated controls, selected among patients observed in the Internal Medicine Department.Methods: A1AT genotyping was carried out using PCR amplification of relevant gene segment was followed by restriction enzyme digestion Taq1. Detection of A1AT gene S and Z alleles was determined through analysis of resulting restriction fragment length polymorphism (RFLP). For GSTM and GSTT genotyping we used Multiplex PCR, followed by gel electrophoresis analysis.Results: The molecular analysis identified the MS genotype in 3 (5,4%) patients with lung cancer and 1 (0,8%) of the controls. The heterozigous MZ state was detected neither among cases nor in controls. The prevalence of GSTM null genotype in lung cancer patients was 49,4% compared to 42,8% of controls, also the prevalence of GSTT null genotype in lung cancer patients was 24,5% compared to 18,2% of controls.Conclusions: Our findings (positive statistical significance),suggest that heritable A1AT and GST status may influence the risk of lung cancer developement. ER -