TY - JOUR T1 - <em>Streptococcus pneumoniae</em>-induced regulation of cyclooxygenase-2 in human lung tissue JF - European Respiratory Journal JO - Eur Respir J SP - 1458 LP - 1467 DO - 10.1183/09031936.00186911 VL - 40 IS - 6 AU - Kolja V. Szymanski AU - Mario Toennies AU - Anne Becher AU - Diana Fatykhova AU - Philippe D. N'Guessan AU - Birgitt Gutbier AU - Frederick Klauschen AU - Frank Neuschaefer-Rube AU - Paul Schneider AU - Jens Rueckert AU - Jens Neudecker AU - Torsten T. Bauer AU - Klaus Dalhoff AU - Daniel Drömann AU - Achim D. Gruber AU - Olivia Kershaw AU - Bettina Temmesfeld-Wollbrueck AU - Norbert Suttorp AU - Stefan Hippenstiel AU - Andreas C. Hocke Y1 - 2012/12/01 UR - http://erj.ersjournals.com/content/40/6/1458.abstract N2 - The majority of cases of community-acquired pneumonia are caused by Streptococcus pneumoniae and most studies on pneumococcal host interaction are based on cell culture or animal experiments. Thus, little is known about infections in human lung tissue. Cyclooxygenase-2 and its metabolites play an important regulatory role in lung inflammation. Therefore, we established a pneumococcal infection model on human lung tissue demonstrating mitogen-activated protein kinase (MAPK)-dependent induction of cyclooxygenase-2 and its related metabolites. In addition to alveolar macrophages and the vascular endothelium, cyclooxygenase-2 was upregulated in alveolar type II but not type I epithelial cells, which was confirmed in lungs of patients suffering from acute pneumonia. Moreover, we demonstrated the expression profile of all four E prostanoid receptors at the mRNA level and showed functionality of the E prostanoid4 receptor by cyclic adenosine monophosphate production. Additionally, in comparison to previous studies, cyclooxygenase-2/prostaglandin E2 related pro- and anti-inflammatory mediator regulation was partly confirmed in human lung tissue after pneumococcal infection. Overall, cell type-specific and MAPK-dependent cyclooxygenase-2 expression and prostaglandin E2 formation in human lung tissue may play an important role in the early phase of pneumococcal infections. ER -