RT Journal Article
SR Electronic
T1 Bone marrow-derived mononuclear cell therapy attenuates silica-induced lung fibrosis
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP 1217
OP 1225
DO 10.1183/09031936.00205009
VO 37
IS 5
A1 T. Maron-Gutierrez
A1 R.C. Castiglione
A1 D.G. Xisto
A1 M.G. Oliveira
A1 F.F. Cruz
A1 R. Peçanha
A1 H. Carreira-Junior
A1 D.S. Ornellas
A1 M.O. Moraes
A1 C.M. Takiya
A1 P.R.M. Rocco
A1 M.M. Morales
YR 2011
UL http://erj.ersjournals.com/content/37/5/1217.abstract
AB This study tests the hypothesis that bone marrow-derived mononuclear cell (BMDMC) therapy may reduce lung inflammation and fibrosis leading to an improvement in respiratory mechanics in a murine model of silicosis. 52 female C57BL/6 mice were randomly assigned into four groups. In the silica group (SIL), silica suspension (20 mg/50 μL in saline) was intratracheally instilled. In the control animals, 50 μL saline was administered intratracheally. At 1 h, the control and SIL groups were further randomised, receiving BMDMC (2×106 i.v. control–cell and SIL–cell) or saline (50 μL i.v. control and SIL). BMDMC were obtained from male donor mice. At day 15, lung mechanics, histology, and the presence of Y chromosome, interleukin (IL)-1β, IL-1α, IL-1 receptor antagonist (IL-1RN), IL-1 receptor type 1, transforming growth factor (TGF)-β and caspase-3 mRNA expressions in lung tissue were analysed. In the SIL–cell group, the fraction area of granuloma, the number of macrophages and the collagen fibre content were reduced, yielding improved lung mechanics. The presence of male donor cells in lung tissue was not confirmed using detection of Y chromosome DNA. Nevertheless, caspase-3, IL-1β, IL-1α, IL-1RN and TGF-β mRNA expression diminished after cell therapy. In conclusion, BMDMC acted on inflammatory and fibrogenic processes improving lung function through paracrine effects.