TY - JOUR T1 - Glucocorticoid receptor-binding characteristics in severe asthma JF - European Respiratory Journal JO - Eur Respir J SP - 985 LP - 988 DO - 10.1183/09031936.03.00059802 VL - 21 IS - 6 AU - C. Bonnans AU - P. Chanez AU - H. Meziane AU - P. Godard AU - J. Bousquet AU - I. Vachier Y1 - 2003/06/01 UR - http://erj.ersjournals.com/content/21/6/985.abstract N2 - The cellular mechanisms associated with severe asthma are still poorly understood. This study investigated the association between glucocorticoid-receptor (GR) alterations and continuous oral glucocorticoid therapy requirement in severe asthma. GR-binding affinity (Kd) and receptor number (n) in peripheral blood monocytes (PBM) obtained from 10 normal subjects, 10 untreated, intermittent asthmatics and 10severe asthmatics were assessed. Moreover, one ability of dexamethasone to inhibit regulated on activation, T-cells expressed and secreted (RANTES) release by these cells in vitro was investigated. GR-binding characteristics were studied in PBM using a 3H dexamethasone ligand-binding assay and Scatchard analysis. RANTES release was measured in the supernatant of PBM at 24 h using an enzyme-linked immunosorbent assay. No significant differences in Kd and n were found between the three groups of patients. Dexamethasone in vitro was able to inhibit RANTES release (mean±sem), with the same concentration/response curve in intermittent, untreated asthmatics (0.47±0.22 versus 1.64±0.31 ng·mL−1) and severe asthmatics (1.49±0.64 versus 2.59±0.77 ng·mL−1). This study showed that, despite long-term treatment with oral glucocorticoids, there was no evidence of abnormalities in glucocorticoid receptor-binding characteristics in severe asthma, and moreover, it was demonstrated that glucocorticoid receptors were functional in vitro. This study was supported by the Direction de la Recherche Clinique, CHU Montpellier, France. ER -