TY - JOUR T1 - Reference equations for pulmonary diffusing capacity of CO and NO in adult Caucasians JF - European Respiratory Journal JO - Eur Respir J DO - 10.1183/13993003.00677-2015 SP - 1500677 AU - Mathias Munkholm AU - Jacob Louis Marott AU - Lars Bjerre-Kristensen AU - Flemming Madsen AU - Ole Find Pedersen AU - Peter Lange AU - Børge G Nordestgaard AU - Jann Mortensen Y1 - 2018/01/01 UR - http://erj.ersjournals.com/content/early/2018/05/24/13993003.00677-2015.abstract N2 - Objective: The aim of this study was to determine reference equations for the combined measurement of carbon monoxide (CO) and nitric oxide (NO) diffusing capacity (DL,CO,NO). In addition, we wanted to appeal for consensus regarding methodology of the measurement including calculation of diffusing capacity of the alveolo-capillary membrane (Dm) and pulmonary capillary volume (Vc).Methods: DL,CO,NO was measured in 282 healthy individuals aged 18–97 years using the single breath technique and a breath-hold time of 5 seconds (true apnoea period). The following values were used: 1) specific conductance of NO (θNO)=4.5 mLNO·mLblood−1·min−1·mmHg−1, 2) ratio of diffusing capacity of the membrane for NO and CO (DmNO/DmCO)=1.97, and 3) 1/red cell CO conductance (1/θCO)=(1.30+0.0041·mean capillary oxygen pressure)·(14.6/Hb concentration in g·dL−1).Results: Reference equations were established for the outcomes of DL,CO,NO including diffusing capacity of CO (DL,CO) and NO (DL,NO) and the calculated values Dm and Vc. Independent variables were age, sex, height and age squared.Conclusion: By providing new reference equations and by appealing for consensus regarding the methodology, we hope to provide a basis for future studies and clinical use of this novel and interesting method.FootnotesThis manuscript has recently been accepted for publication in the European Respiratory Journal. It is published here in its accepted form prior to copyediting and typesetting by our production team. After these production processes are complete and the authors have approved the resulting proofs, the article will move to the latest issue of the ERJ online. Please open or download the PDF to view this article.Conflict of interest: Dr. Munkholm reports grants from Danish Lung Association, grants from Boehringer Ingelheim, grants from GlaxoSmithKline, grants from Novartis, grants from Alere, non-financial support from Intra Medic, during the conduct of the study;.Conflict of interest: Dr. Bjerre-Kristensen reports grants from Danish Lung Association, grants from Boehringer Ingelheim, grants from GlaxoSmithKline, grants from Novartis, grants from Alere, non-financial support from Intra Medic, during the conduct of the study;.Conflict of interest: Dr. Madsen reports grants from Allergy And Lung Clinic Helsingør, during the conduct of the study;.Conflict of interest: Dr. Pedersen reports grants from Danish Lung Association, grants from Boehringer Ingelheim, grants from GlaxoSmithKline, grants from Novartis, grants from Alere, non-financial support from Intra Medic, during the conduct of the study;.Conflict of interest: Dr. Lange reports grants from Danish Lung Association, grants from Boehringer Ingelheim, grants from GlaxoSmithKline, grants from Novartis, grants from Alere, non-financial support from Intra Medic, during the conduct of the study;.Conflict of interest: Dr. Nordestgaard has nothing to disclose.Conflict of interest: Dr. Mortensen reports grants from Danish Lung Association, grants from Boehringer Ingelheim, grants from GlaxoSmithKline, grants from Novartis, grants from Alere, non-financial support from Intra Medic, during the conduct of the study;.Conflict of interest: Mr. Marott reports grants from Danish Lung Association, grants from Boehringer Ingelheim, grants from GlaxoSmithKline, grants from Novartis, grants from Alere, non-financial support from Intra Medic, during the conduct of the study;. ER -