RT Journal Article SR Electronic T1 Impact of the mitochondria-targeted antioxidant MitoQ on hypoxia-induced pulmonary hypertension JF European Respiratory Journal JO Eur Respir J FD European Respiratory Society SP 1701024 DO 10.1183/13993003.01024-2017 VO 51 IS 3 A1 Oleg Pak A1 Susan Scheibe A1 Azadeh Esfandiary A1 Mareike Gierhardt A1 Akylbek Sydykov A1 Angela Logan A1 Athanasios Fysikopoulos A1 Florian Veit A1 Matthias Hecker A1 Florian Kroschel A1 Karin Quanz A1 Alexandra Erb A1 Katharina Schäfer A1 Mirja Fassbinder A1 Nasim Alebrahimdehkordi A1 Hossein A. Ghofrani A1 Ralph T. Schermuly A1 Ralf P. Brandes A1 Werner Seeger A1 Michael P. Murphy A1 Norbert Weissmann A1 Natascha Sommer YR 2018 UL http://erj.ersjournals.com/content/51/3/1701024.abstract AB Increased mitochondrial reactive oxygen species (ROS), particularly superoxide, have been suggested to mediate hypoxic pulmonary vasoconstriction (HPV), chronic hypoxia-induced pulmonary hypertension and right ventricular remodelling.We determined ROS in acute and chronic hypoxia, and investigated the effect of the mitochondria-targeted antioxidant MitoQ under these conditions.The effect of MitoQ or its inactive carrier substance, decyltriphenylphosphonium, on acute HPV (1% O2 for 10 min) was investigated in isolated blood-free perfused mouse lungs. Mice exposed to chronic hypoxia (10% O2 for 4 weeks) or after banding of the main pulmonary artery were treated with MitoQ or decyltriphenylphosphonium (50 mg·kg−1·day−1).Total cellular superoxide and mitochondrial ROS levels were increased in pulmonary artery smooth muscle cells but decreased in pulmonary fibroblasts in acute hypoxia. MitoQ significantly inhibited HPV and acute hypoxia-induced rise in superoxide concentration. ROS was decreased in pulmonary artery smooth muscle cells, while it increased in the right ventricle after chronic hypoxia. Correspondingly, MitoQ did not affect the development of chronic hypoxia-induced pulmonary hypertension but attenuated right ventricular remodelling after chronic hypoxia as well as after pulmonary arterial banding.Increased mitochondrial ROS of pulmonary artery smooth muscle cells mediate acute HPV, but not chronic hypoxia-induced pulmonary hypertension. MitoQ may be beneficial under conditions of exaggerated acute HPV.The mitochondria-targeted antioxidant MitoQ attenuates acute hypoxic pulmonary vasoconstriction and RV remodelling, but not chronic hypoxia-induced PH http://ow.ly/PCCU30hSJKK