RT Journal Article
SR Electronic
T1 Indoor bacteria and asthma in adults: a multicentre case–control study within ECRHS II
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP 1701241
DO 10.1183/13993003.01241-2017
VO 51
IS 2
A1 Juha Pekkanen
A1 Maria Valkonen
A1 Martin Täubel
A1 Christina Tischer
A1 Hanna Leppänen
A1 Päivi M. Kärkkäinen
A1 Helena Rintala
A1 Jan-Paul Zock
A1 Lidia Casas
A1 Nicole Probst-Hensch
A1 Bertil Forsberg
A1 Mathias Holm
A1 Christer Janson
A1 Isabelle Pin
A1 Thorarinn Gislason
A1 Debbie Jarvis
A1 Joachim Heinrich
A1 Anne Hyvärinen
YR 2018
UL http://erj.ersjournals.com/content/51/2/1701241.abstract
AB Both protective and adverse effects of indoor microbial exposure on asthma have been reported, but mostly in children. To date, no study in adults has used non-targeted methods for detection of indoor bacteria followed by quantitative confirmation.A cross-sectional study of 198 asthmatic and 199 controls was conducted within the European Community Respiratory Health Survey (ECRHS) II. DNA was extracted from mattress dust for bacterial analysis using denaturing gradient gel electrophoresis (DGGE). Selected bands were sequenced and associations with asthma confirmed with four quantitative PCR (qPCR) assays.15 out of 37 bands detected with DGGE, which had at least a suggestive association (p<0.25) with asthma, were sequenced. Of the four targeted qPCRs, Clostridium cluster XI confirmed the protective association with asthma. The association was dose dependent (aOR 0.43 (95% CI 0.22–0.84) for the fourth versus first quartile, p for trend 0.009) and independent of other microbial markers. Few significant associations were observed for the three other qPCRs used.In this large international study, the level of Clostridium cluster XI was independently associated with a lower risk of prevalent asthma. Results suggest the importance of environmental bacteria also in adult asthma, but need to be confirmed in future studies.Microbial exposures at home may also protect from asthma in adults, not only in children http://ow.ly/7cnI30hzSox