RT Journal Article
SR Electronic
T1 Interplay between cigarette smoking and pulmonary reverse lipid transport
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP 1700681
DO 10.1183/13993003.00681-2017
VO 50
IS 3
A1 Éric Jubinville
A1 Maude Talbot
A1 Jean-Christophe Bérubé
A1 Mélanie Hamel-Auger
A1 Michaël Maranda-Robitaille
A1 Marie-Josée Beaulieu
A1 Sophie Aubin
A1 Marie-Ève Paré
A1 David G. Kallend
A1 Benoit Arsenault
A1 Yohan Bossé
A1 Mathieu C. Morissette
YR 2017
UL http://erj.ersjournals.com/content/50/3/1700681.abstract
AB Reverse lipid transport is critical to maintain homeostasis. Smoking causes lipid accumulation in macrophages, therefore suggesting suboptimal reverse lipid transport mechanisms. In this study, we investigated the interplay between smoking and reverse lipid transport and the consequences on smoking-induced lung and peripheral alterations.To investigate the relationship between smoking and reverse lipid transport, we used a clinical lung gene expression dataset and a mouse model of cigarette smoke exposure. We also used ApoA-1−/− mice, with reduced reverse lipid transport capacity, and a recombinant ApoA-1 Milano/phospholipid complex (MDCO-216) to boost reverse lipid transport. Cellular and functional analyses were performed on the lungs and impact on body composition was also assessed.Smoking affects pulmonary expression of abca1, abcg1, apoe and scarb1 in both mice and humans, key genes involved in reverse lipid transport. In mice, the capacity of bronchoalveolar lavage fluid and serum to stimulate cholesterol efflux in macrophages was increased after a single exposure to cigarette smoke. ApoA-1−/− mice showed increased lung neutrophilia, larger macrophages and greater loss in lean mass in response to smoking, whereas treatment with MDCO-216 reduced the size of macrophages and increased the lean mass of mice exposed to cigarette smoke.Altogether, this study shows a functional interaction between smoking and reverse lipid transport, and opens new avenues for better understanding the link between metabolic and pulmonary diseases related to smoking.Smoking affects reverse lipid export mechanisms, represent a new pathological mechanism and therapeutic target http://ow.ly/g8pw30dWU8U