PT  - JOURNAL ARTICLE
AU  - Marcin Maciej Kurowski
AU  - Janusz Jurczyk
AU  - Malgorzata Pawelczyk
AU  - Aleksandra Szulc
AU  - Hubert Krysztofiak
AU  - Marek L. Kowalski
TI  - Treadmill exercise decreases expression of innate immunity molecules in peripheral blood leukocytes in competitive athletes, asthmatics and healthy subjects
AID  - 10.1183/13993003.congress-2016.PA571
DP  - 2016 Sep 01
TA  - European Respiratory Journal
PG  - PA571
VI  - 48
IP  - suppl 60
4099  - http://erj.ersjournals.com/content/48/suppl_60/PA571.short
4100  - http://erj.ersjournals.com/content/48/suppl_60/PA571.full
SO  - Eur Respir J2016 Sep 01; 48
AB  - Background: Intensive exercise may affect the innate immunity, influence inflammatory response and increase susceptibility to respiratory infections.Aim: To assess if exercise modifies expression of innate immune response molecules in peripheral blood leukocytes (PBL) of competitive athletes, asthmatics and healthy subjects.Methods: Study included 15 athletes (speed skaters and swimmers), 9 mild-to-moderate asthmatics (AC) and 7 healthy non-exercising subjects (HC).Treadmill exercise challenge was done and PBLs were isolated before, immediately after and 1 hour post-exercise. Following RNA isolation relative gene expression was assessed by qPCR and calculated using 2-Δ ΔCt method with pre-exercise expression as reference.Results: Significant decrease in mRNA expression immediately post-exercise was observed for IL-1ra in athletes (mean decrease 72%;p<0.02) and HC (mean decrease 66%;p<0.05) and for HSPA1 in athletes (61%;p<0.01) and HC (58%;p=0.03). TNF-α gene expression decreased immediately after and 1 hour after exercise in athletes (63%;p<0.01 and 47%;p<0.05, respectively) and HC (56% and 48%; p<0.05). CD14 expression was significantly decreased in all groups at both time-points.Conclusions: Acute exercise decreases innate immune response in PBLs to similar extent in competitive athletes and healthy subjects.