RT Journal Article SR Electronic T1 Comparative lentiviral versus adenoviral gene transfer of GM-CSF to bone marrow-derived versus pulmonary macrophages of mice JF European Respiratory Journal JO Eur Respir J FD European Respiratory Society SP PA2748 DO 10.1183/13993003.congress-2015.PA2748 VO 46 IS suppl 59 A1 Alena Singpiel A1 Julia Schneider A1 Regina Maus A1 Jennifer Bohling A1 Friederike Behler-Janbek A1 Renata Stripecke A1 Tobias Welte A1 Ulrich Maus YR 2015 UL http://erj.ersjournals.com/content/46/suppl_59/PA2748.abstract AB Granulocyte macrophage colony stimulating factor (GM-CSF) is a hematopoietic growth factor involved in the terminal differentiation and survival of alveolar macrophages with important implications for macrophage-dependent lung antibacterial immunity. We here assessed the transduction efficacy of murine bone marrow-derived monocytes (BMM) versus resident alveolar macrophages (rAM) employing either lentiviral or adenoviral vectors encoding for murine GM-CSF (LV-GM-CSF versus AdGM-CSF). Lentiviral transduction of BMM for 16 hours resulted in increased transgene and protein expression beginning at day 3 until day 28, with significantly improved survival of LV-GM-CSF as compared to LV-eGFP transduced BMM. In contrast, rAM demonstrated substantial cytotoxic cell death upon lentiviral transduction compared to BMM. In contrast, transfection of rAM with AdGM-CSF did not cause any cytotoxicity, and resulted in peak transgene and protein expression on day 7 post-transfection with a decline thereafter. These data reveal different efficacy profiles of viral vector systems in terms of transgene expression and cytotoxicity in pre-differentiated as compared to terminally differentiated macrophage populations.