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Supplementary material
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- Supplementary material -
Materials and methods
Results - Figure S1 - Percentages of TNF (A) or IL-2 (B) expressing BAL and blood CD4pos or CD8pos T cells of L�fgren�s patients in response to mKatG or PPD (media alone or unstimulated whole blood were used as negative control; superantigens Staphylococcus enterotoxin A (SEA) and SEB were used as positive control). The statistical analyses were done using raw data, i.e. background (cytokine production in BAL after incubation in media alone, or in unstimulated whole blood) was not deducted. Horizontal lines depict median percentage of cytokine production. Wilcoxon signed rank test, * p<0.05, ** p<0.01, *** p<0.001.
- Figure S2 - T cell responses to mycobacterial proteins in patients without L�fgren�s syndrome. Percentages of IFNγ-expressing BAL and blood CD4pos or CD8pos T cells (A), or TNF-expressing CD4pos or CD8pos T cells (B), in response to mKatG or PPD (media alone or unstimulated whole blood were used as negative control; superantigens Staphylococcus enterotoxin A (SEA) and SEB were used as positive control). The statistical analyses were done using raw data, i.e. background (cytokine production in BAL after incubation in media alone, or in unstimulated whole blood) was not deducted. Comparison between BAL and blood regarding the percentages of IFNγ-expressing CD4pos or CD8pos T cells after stimulation with mKatG or PPD (C). Comparison between patients with or without L�fgren�s syndrome regarding total IFNγ or TNF production in BAL and blood CD4pos T cells in response to antigen stimulation (D). The statistical analyses in (C) and (D) were done after deduction of background. Horizontal lines depict median percentage of cytokine production. Wilcoxon signed rank test, * p<0.05, ** p<0.01, *** p<0.001.
- Figure S3 - Spontaneous cytokine expression. Percentages of IFN, TNF and IL-2 producing cells in CD4+ subsets (AV2S3pos and AV2S3neg) in BAL cells incubated in media alone (A) or in unstimulated blood cells (B). Comparison between BAL and blood regarding the percentage of IFNγ-expressing TCR AV2S3pos and AV2S3neg CD4pos T cells after antigen stimulation (C). Percentage of CD4pos T cells responding to mKatG or PPD with IFNγ or TNF production, respectively, that are AV2S3pos (D). The statistical analyses were done after deduction of background. Horizontal lines depict median percentage of cytokine production. Wilcoxon signed rank test, * p<0.05, ** p<0.01, n.s.=not significant.
- Figure S4 - Median fluorescent intensity (MFI) for IFNγ and TNF, respectively, of single IFNγ, single TNF, or simultaneous IFNγ and TNF (i.e. multifunctional) production in blood CD4pos T cells, after stimulation with mKatG or PPD. The statistical analyses were done after deduction of background. Horizontal lines depict median percentage of cytokine production. Wilcoxon signed rank test, * p<0.01, *** p<0.001.
- Supplementary material -